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Table 1: Average degradation rate from BOD and from direct determination (LC analysis) of the test substance and main degradation substance.
Degradation rate (%)
Average degradation rate
of the test substance
( LC )
of the main impurity of the test substance
*11 From results of LC analysis of the main impurity of the test substance, its amount in test solutions after the exposure was larger than that added at the beginning. The main impurity of the test substance was estimated to be produced by the transformation of the test substance. Therefore, the amount of the main impurity at the beginning was assumed to fully remain during the exposure time (residual amount = amount added at the beginning), the degradation rate from LC analysis of the main impurity was calculated by using this assumed residual amount.
Table 2. Residual rates (%), production rates (%) and material balance of test substance, main impurity and main degradation products of the test substance.
Residual rate (%)
Production rate (%)
degradation product 1
degradation product 2
Material balance – 1 (%)
Material balance – 2 (%)
Degr. product 3
Degr. product 4
Degr. product 5
N°: Channel number of detection unit of coulometer
Production rate: (Production amount of each degradation product) / (Its theoretical [maximum] production amount by the degradation of test substance amount added at the beginning) x 100
Material balance-1: (The summation of residual amount [test substance and main impurity] and production amount*14[main impurity, degradation product 1, degradation product 2, degradation product 3, degradation product 4, and degradation product 5]) / (Test substance [test substance and main impurity] amount added at the beginning) x 100
Material balance-2: (The summation of the above residual and production*14amount and that of mineralized
degradation product 2 equivalent to DOC degradation rate of 5%) / (Test substance amount added at the beginning) x 100
*12 Theoretical [maximum] production amount of degradation products 1 and 2 was calculated on the assumption that they were derived from the hydrolysis of only the test substance, although they were considered to be produced from both the test substance andthe impurity of the test subtance.
*13 Since the degradation products 3, 4 and 5 were difficult to obtain as the analytical standards, the test substance was used as the analytical standard for quantitative analysis of these products on the assumption that each weight adsorption coefficient should be equal to that of the test substance.
*14 Because the production of the degradation product 1 and 2 by hydrolysis results in the addition of water molecule, the material balance will be overestimated by using the values (not corrected) of production amount of degradation products 1 and 2. Therefore, in order to avoid the overestimation due to the addition by water molecule, the material balance was evaluated with the correction taking it into account.
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