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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From November 2012 to January 2013
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study (OECD, EPA, etc)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Qualifier:
according to
Guideline:
EPA OPPTS 850.6200 (Earthworm Subchronic Toxicity Test)
Qualifier:
according to
Guideline:
other: GBff 21809-2008. Chemicals, Earthwonn, Acute Toxicity Test.
Qualifier:
according to
Guideline:
other: SEPA, the Guidelines for Hazard Evaluation of New Chemical Substances, Environmental Protection Agency ofP.R.C. (HJ/T 154-2004)
GLP compliance:
yes (incl. certificate)
Remarks:
GLP compliance statement

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: white solid
Details on test material:
- Name of test material (as cited in study report): XP-7866
- Physical state: white solid
- Analytical purity: 99.5 wt%
- Purity test date: 99.5 wt%
- Lot/batch No.: 10188-1JM
- Expiration date of the lot/batch: 28th February, 2015
- Storage condition of test material: container closed at room temperature (15-25°C)

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
Analytical monitoring not performed

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: obtained from Chenggong Earthworm Raising Farm located in Dongli District, Tianjing. (earthworm20121121)
- Age at test initiation (mean and range, SD): healthy adult (more than two months old with clitellum)
- Weight at test initiation (mean and range, SD): 302 to 598 mg.

ACCLIMATION
For each test container, ten earthwonns, which had been conditioned for 24 hours in an artificial soil

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d

Test conditions

Test temperature:
19.25°C to 21.70°C
pH:
6.27
Moisture:
15.5%
NOTE: Moisture content was detennined before the test by drying a small sample at 105°C for at least 3 hours and re-weighing.
Details on test conditions:
TEST SYSTEM
- Test container (material, size): Artificial climate incubator: Shanghai BoXun BIC-400. (height: 19 cmXlength: 12 cm X width: 9 cm)
NOTE: The containers were covered with a glass board to prevent the earthwonns from escaping and the test medium from drying and kept under the test conditions for 14 days.
- Amount of soil or substrate:
* Proper weight of test item (2.4068 g) was weighed. Then 50 g of fine ground quartz was added and mixed fully. The mixture of quartz and test item was added into 2749.9 g wet artificial soil (dry weight was 2350 g). After mixing, 400 ml of distilled water was added to adjust the moisture content to ca. 33% of dry weight. Then all the components were mixed again, at last, 750 g (749.7 g-750.3 g) of the test medium was weighed and put into its corresponding labeled test container.
* The control groups were prepared under identical procedures but not exposed to the test item.
- No. of organisms per container (treatment): 10 worms per replicates
- No. of replicates per treatment group: 4 replicates
- No. of replicates per control: 4 replicates


ARTIFICIAL SOIL:
* sphagnum peat moss 10% (the moisture content was detennined as 15.5%)
* kaolin clay 20%;
* quartz sand 68%;
* calcium carbonate 2%.
The above constituents were blended in a laboratory mixer. Distilled water was added to adjust the moisture.

OTHER TEST CONDITIONS
- Photoperiod: light kept continuous
- Light intensity: in the range of 463 Lux to 708 Lux.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
* Mortality
* Observed behavioral or pathological symptoms
* individual weights of all surviving wonns

VEHICLE CONTROL PERFORMED: No vehicle used in the experiment

TEST CONCENTRATIONS
One dose only 1000 mg/kg dry soil weight
Nominal and measured concentrations:
1000 mg/kg dry soil weight

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- No mortality and toxic symptoms were found in both control group and test groups during the test.
- Weight of Earthworms at the end of the test:
* Control: 436 mg +/- 78
* 1000: 405 mg +/- 84
- Validity of the test:
The mortality in the control did not exceed 10 percent at the end of the test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the test results and the eco-toxicity grading criteria in the Guidelines for Hazard Evaluation of New Chemical Substances, Environmental Protection Agency of P.R.C. (HJ/T 154-2004), the test item is graded as a chemical with low acute toxicity (0) to earthworms under the experimental conditions of this study. Indeed, the LC50 was determined to be higher than 1000 mg/kg dry wt of this limit test and therefore the NOEC was determined to be 1000 mg/kg dry wt.