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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12-Jan-2004 to 16-Jan-2004
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP-compliant, guideline study, available as an unpublished report, acceptable with restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Pyrolysis C10+ Fuel Oil, CASRN 68513-69-9 and 68921-67-5
Liquid under ambient laboratory conditions and immiscible in water and methanol but miscible in hexane. Initial density was measured (at 20°C) as 0.972 g/ml and final density was 0.973 g/ml.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples taken (with no headspace) Day 0, Day 1, Day 3 and Day 4, prior to renewals and in a composite of the replicates after each renewal. Observations were performed at 3, 6, 24, 48, 72 and 96 hrs after the beginning of the test

Test solutions

Vehicle:
no
Details on test solutions:
Treatments prepared by adding the appropriate amount of substance to 18.0L of dilution water in glass aspirator bottles. The mixtures were stirred for 24hrs. All treatments appeared clear/colourless with the test substnace floating at the surface. Mixtures were cooled for one hour without stirring before WAFs were removed.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Thomas Fish Company, Anderson, CA, USA. Lot 482. Receipt date: 30-Dec-03
For the 13 days prior to use the fish were observed for disease and parasites (for which they had not previously been treated). They were kept at approximately 14.1 °C in dilution water which was aerated continuously to provide a DO concentration of at least 80%. They were held under filtrated static conditions and were fed with Finfish Starter at least 5 days per week. There were no observed mortalities.
72 fish, 29 days post hatch. Mean weight: 0.206 g. Mean total length: 3.1 cm.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
none

Test conditions

Hardness:
104 mg/L as CaCO₃
Test temperature:
13.6 °C (mean) (S.D.=0.1)
pH:
7.3 to 8.1
Dissolved oxygen:
6.8 to 8.6 mg/L
Salinity:
NA
Nominal and measured concentrations:
0, 0.40, 0.79, 1.7, 3.7 and 6.3 (mg/L, measured) plus the control
Actual loading rate (mg/l): 0, 0.47, 0.90, 1.8, 3.5 and 7.0
Details on test conditions:
Reconstituted UV-sterilized, deionized, well water (Batch 290)
4L aspirator bottles containing approx 4.5L test solution (no headspace)
Aeration
Daily renewal
4 organisms per vessel
3 replicates per concentration
Biomass loading: 0.183 g of fish/L
16 hours light; 8 hours dark
607 to 614 Lux during full daylight periods.
Fish were not fed during the study, nor for 24 hours prior to commencement.

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
1.1 mg/L
Nominal / measured:
nominal
Conc. based on:
other: actual loading rate
Basis for effect:
mortality
Remarks on result:
other: 95% CL 1.0-1.3
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% 0.9-1.2
Details on results:
Results have been expressed as the lethal loading/lethal concentration which result in 50% mortality compared to the control for the specified time of exposure
Results with reference substance (positive control):
na
Reported statistics and error estimates:
LL50 and LC50 (24-96 hour) were determined using a Trimmed Spearman-Karber Method.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96-hour LC50 was reported to be 1.0 mg/L.
Executive summary:

The 96-hour LL50 was reported to be 1.1 mg/L. whilst the LC50 was 1mg/L. This study is GLP compliant and follows a standard guideline. It is considered reliable and suitable for use as a key study.