Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 701-124-4 | CAS number: -
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Falling short of highest standards concerning aspects of protocol or reporting. Acceptable literature publication. Useful: supporting data.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Untersuchungen zur Stabilitat aromatischer Diisocyanat in Dimethylsulphoxid |(DMSO): Toluylendiisocyanat (TDI) und Diphenylmethandiisocyanat (MDI) im Ames-Test
- Author:
- Gahlmann R, Herbold B, Ruckes A & Seel K
- Year:
- 1 993
- Bibliographic source:
- Zbl.Arbeitsmed. 43: 34-38
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Principles of method if other than guideline:
- Pre-dates, but is similar to, those of 84/449/EEC, B12, and OECD Test Guideline 474.
- GLP compliance:
- no
- Type of assay:
- micronucleus assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Type:
- Constituent
- Details on test material:
- 4,4'-diphenylmethane diisocyanate in dimethyl sulphoxide,
suspended in corn oil before use.
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- not specified
Administration / exposure
- Route of administration:
- intraperitoneal
- Duration of treatment / exposure:
- 24 hour
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0-200 mg MDI/kg
Basis:
Results and discussion
Test results
- Sex:
- not specified
- Additional information on results:
- An acute toxicity test gave a 7 day LD50 of about 100 mg/kg.
There were no deaths within 24 hr.
It was concluded that the test substance did not cause micronuclei, and therefore did not induce in vivo chromosomal aberration.
The number of erythrocytes with micronuclei differed little between treated groups and the solvent control.
The only statistically significant decrease in polychromatic erythrocytes occurred when the test substance was used at 200 mg/kg. This was taken to suggest inhibited myelopoietic function of the bone marrow.
When compared with distilled water, dimethyl sulphoxide in corn oil gave a statistically insignificant reduction in polychromatic erythrocytes, and increase in erythrocytes with micronuclei.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Route: .live1