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Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with national standard methods with acceptable restrictions (only one sex and one concentration tested, no determination of LC50 possible)

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
no guideline followed
Principles of method if other than guideline:
Six mature male rats were placed in a stainless steel chamber of 35-liters capacity and exposed for six hours to a concentrated atmosphere of vapors produced by passing a stream of air through 125.2 grams of the compound contained in a 500-milliliter erlenmeyer flask. The sample was maintained at a temperature of 150° F (65.6° C) by immersing the flask in a heated oil bath. Vapors from the flask passed into a one liter bottle to remove droplets and then into the chamber. Air flow through the sample was 4.0 liters per minute as measured by a calibrated rotameter. No supplementary air was introduced inasmuch as the above supply was ample for the animals' oxygen requirements. The animals were observed for behavior during exposure and for 14 days following exposure. The viscera of the test animals were examined macroscopically.
GLP compliance:
Test type:
standard acute method

Test material

Constituent 1
Reference substance name:
Cas Number:

Test animals

not specified
Details on test animals or test system and environmental conditions:
no data

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
other: air
Details on inhalation exposure:
see "Principles of method"
Analytical verification of test atmosphere concentrations:
Amount of sample: to start (125.2 g), revovered (124.5 g), total vaporized (0.7 g), recovered from condenser (0.0 g), weight of vapors entering chamber (0.7 g), average concentration of vapors in chamber (0.48 g/m3)
Duration of exposure:
6 h
mean concentration: 480 mg/m3
No. of animals per sex per dose:
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: not specified
- Necropsy of survivors performed: yes (viscera only)
- Other examinations performed: clinical signs, body weight
no data

Results and discussion

Effect levels
Dose descriptor:
Effect level:
> 480 mg/m³ air (analytical)
Exp. duration:
6 h
All six animals survived the 6-hr exposure as well as the following 14-day observation period.
Clinical signs:
other: Upon removal from the chamber respiration was normal and there were no other signs of toxic distress. The balance of the observation period was uneventful.
Body weight:
Weight gain was normal.
Gross pathology:
The viscera appeared normal by macroscopic examination.
Other findings:
no data

Applicant's summary and conclusion

Executive summary:

The acute inhalation toxicity of the test substance revealed an LC50 value > 480 mg/m3 for male rats. An 6-hr vapor exposure of 480 mg/m3 was tolerated without mortality and without signs of intoxication by all animals. Body weight development was not affected (Birch, 1977). Due to the low test concentration of 480 mg/m3 test substance vapor this study is considered to be of limited value for classification.