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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data from secondary source

Data source

Referenceopen allclose all

Reference Type:
other: Authoritative database
Title:
Acute dermal toxicity for CAS NO: 107-64-2
Author:
IFA GESTIS
Year:
2017
Bibliographic source:
GESTIS SUBSTANCE Database (information system in hazardous substance of the Berufsgenossenscheftn), 2017
Reference Type:
secondary source
Title:
Initial Submission: Letter from Monsanto Co to USEPA regarding Cyanuric Acid Crude with Attachments and Cover Letter Dated 072392
Author:
NTRL
Year:
1992
Bibliographic source:
NTRL report, OTS0538650, 1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
other: As mentioned below
Principles of method if other than guideline:
An acute dermal toxicity study of Cyanuric acid was carried out on rabbits
GLP compliance:
not specified
Test type:
other: not specified
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material: Cyanuric acid
- IUPAC name: 1,3,5-Triazinane-2,4,6-trione
- Molecular formula: C3H3N3O3
- Molecular weight: 129.075 g/mol
- Substance type: Organic
Specific details on test material used for the study:
- Name of test material: Cyanuric acid
- IUPAC name: 1,3,5-Triazinane-2,4,6-trione
- Molecular formula: C3H3N3O3
- Molecular weight: 129.075 g/mol
- Substance type: Organic

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female
Details on test animals and environmental conditions:
Details on test animal
TEST ANIMALS
- Source: Isf:(NZW), Isaac's Farm, Litchfield, Illinois)
- Age at study initiation:
- Weight at study initiation:2.51-2.79kg
- Fasting period before study: No data available
- Housing: Each animal was identified by ear tag and bar coded cage card.
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data available
- Humidity (%):No data available
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available
IN-LIFE DATES: From: To:

Administration / exposure

Type of coverage:
occlusive
Vehicle:
physiological saline
Details on dermal exposure:
DOSAGE PREPARATION (if unusual): The test material, ground with a mortar and pestle and moistened with physiological saline,
TEST SITE
- Area of exposure: Dorsal surface
- % coverage: no data
- Type of wrap if used: occlusive wrap of latex rubber
secured by bandaging and elastic tape


REMOVAL OF TEST SUBSTANCE
- Washing (if done): material was wiped from the animal.
- Time after start of exposure:24hr

TEST MATERIAL
- Amount(s) applied (volume or weight with unit):5000mg/kg bw
- Concentration (if solution): No data available
- Constant volume or concentration used: yes/no
- For solids, paste formed: yes,The test material, ground with a mortar and
pestle and moistened with physiological saline


VEHICLE
- Amount(s) applied (volume or weight with unit):No data available
- Concentration (if solution):No data available
- Lot/batch no. (if required): No data available
- Purity:No data available
Duration of exposure:
24hr(test material was held in place by means of an occlusive wrap of latex rubber secured by bandaging and elastic tape. The occlusive wrap was removed after approximately 24 hours and the excess material was wiped from the animal.)
Doses:
5000mg/kg
No. of animals per sex per dose:
5 male and 5 female
Control animals:
not specified
Details on study design:
Details on study design
- Duration of observation period following administration: 15 days
- Frequency of observations and weighing: Clinical observations were made three times during the first eight hours following test
material administration and twice daily (morning and afternoon) thereafter, with the exceptions of one day when observations were only recorded once and another day when
Observations were only recorded once for nine of the animals. Body weights were recorded on days a (day of exposure), 7, and 14.

- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, were done
other:
Statistics:
No data available

Results and discussion

Preliminary study:
No data available
Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
5 000 mg/kg bw
Based on:
test mat.
Remarks on result:
other: No mortality was observed
Mortality:
No mortality was observed
Clinical signs:
No clinical abnormalities were observed.
Body weight:
No data available
Gross pathology:
At necropsy, one male animal had pale colored kidneys, one female animal had kidneys with pale, mottled exteriors, and another female had tapeworm cysts in the mesentery.None of these effects were attributed to toxicity of the test material.
Other findings:
No data available

Applicant's summary and conclusion

Interpretation of results:
other: Not classified
Conclusions:
The LD 50 was considered to be >5000 mg/kg .When rabbits treated with Cyanuric acid (108-80-5) by dermal application.
Executive summary:

An acute dermal toxicity study of Cyanuric acid(108-80-5) was carried out on New Zealand White rabbits. A group of 5 male and 5 female rabbits were given a single dose of 5000mg/kg bw test materialground with a mortar and pestle and moistened with physiological saline, on the dorsal Surface. Before application the skin on the dorsal surface of each animal was shaved with an electric clipper and abraded with a hypodermic needle.Test materialwas held in place by means of an occlusive wrap of latex rubber secured by bandaging and elastic tape. The occlusive wrap was removed after approximately 24 hoursand the excess material was wiped from the animal. Clinical observations were made three times during the first eight hours following test material administration and twice daily (morning and afternoon) thereafter, with the exceptions of one day when observations were only recorded once and another day when observations were only recorded once for nine of the animals. Body weights were recorded on days a (day of exposure), 7, and 14.No mortality was observed at dose 5000mg/kg bw.At necropsy, one male animal had pale colored kidneys, one female animal had kidneys with pale, mottled exteriors, and another female had tapeworm cysts in the mesentery. None of these effects were attributed to toxicity of the test material. Also no clinical abnormalities were observed.Therefore LD 50 was considered to be >5000 mg/kg .When rabbits treated with Cyanuric acid(108-80-5) by dermal application.