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Short-term toxicity to aquatic invertebrates

Using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate was predicted 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7). Effect concentration i.e EC50 value was estimated to be 540.01 mg/l for daphnia magna for 48 hrs duration. It was concluded that the 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7) was likely to be not toxic to aquatic invertebrate, hence it can be considered to be “not Classified” as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae was predicted 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7). Effect concentration i.e EC50 value was estimated to be 386.95 mg/l for Scenedesmus sp. for 72 hrs duration. It was concluded that the 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7) was likely to be not toxic to aquatic algae, hence it can be considered to be “not Classified” as per the CLP classification criteria for aquatic environment.

Additional information

Short-term toxicity to aquatic invertebrates

Following three studies includes predicted data and experimental data of target chemical and read across chemical to conclude the

4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7) toxicity extent towards the aquatic invertebrate is summarized below :

Using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate was predicted 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7). Effect concentration i.e EC50 value was estimated to be 540.01 mg/l for daphnia magna for 48 hrs duration. It was concluded that the 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7) was likely to be not toxic to aquatic invertebrate, hence it can be considered to be “not Classified” as per the CLP classification criteria for aquatic environment.

The above predicteddata is supported by the experimental data of read across chemical Phloroglucinol (CAS:108-73-6 ) from Gesunds 1960, suggegts that the Short-term toxicity to aquatic invertebrates test was carried for 48 Hour(s) to study the effect of Phloroglucinol on aquatic environment.

The Lethal dose LD50 to 50% ofDaphnia magna at 48 hr at is 600 mg/l. It can be from the value that the Phloroglucinol is not toxic to the aquatic environment.

Similar experimental study for another read across chemicalmolybdenum trioxide (CAS: 1313-27-5) from Department of Entomology, Fisheries and Wildlife 1978, indicates that the Short-term toxicity to aquatic invertebrates test was carried for 48 h under static condition to study the effects of Molybdenum trioxide on aquatic environment.Mortality was observed as effect measurement.

The Lethal concentration LC50 to 50% ofDaphnia magna at 48 hr is 430 mg/l . It can be concluded form the value that the Molybdenum trioxide is not toxic to the aquatic environment.

Thus based on the effect concentrations which is in the range 430 mg/l to 600 mg/l give the conclusion that test substance 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7) was likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and applying the weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following studies includes predicted data and exepimental data of target chemical and stucturally similar read across to conlcude the

4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7) toxicity extent towards aquatic algae is summarized as follows:

Using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae was predicted 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7). Effect concentration i.e EC50 value was estimated to be 386.95 mg/l for Scenedesmus sp. for 72 hrs duration. It was concluded that the 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7) was likely to be not toxic to aquatic algae, hence it can be considered to be “not Classified” as per the CLP classification criteria for aquatic environment.

The predicted study of target chemical is supported by the experimental data of read across chemical Cerium dioxide( CAS: 1306-38-3) from Toxicological sciences 2011, indicates that the Toxicity to aquatic algae and cyanobacteria test was carried out for 24 hr under static condition to study the effects of Cerium dioxide on aquatic environment.Decreasing trend ofPhysiology (Bioluminescence) was considered as effect measurement.The Effective concentration to 50% ofAnabaena sp. At 24 Hour is 100 mg/l.It can be concluded that the Cerium oxide is not toxic to the aquatic environment.

Thus based on the effect concentrations which is in the range 100 mg/l to 386.95 mg/l give the conclusion that test substance 4-hydroxy-6-[(3-sulfophenyl)amino]naphthalene-2-sulfonic acid (CAS: 25251-42-7) was likely to be not toxic to aquatic algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria.