1,8-dihydroxy-4-nitro-5-(phenylamino)anthraquinone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Inhouse method: A-11 devloped by CIBA and is comparable to standard guidline

Deviations:

not specified

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

See any other information on materials and methods below.

Vehicle:

yes

Details on test solutions:

300 and 1000 mg/l

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

See any other information on materials and methods below.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

None

Post exposure observation period:

See any other information on materials and methods below.

Hardness:

None

Test temperature:

23 °C

pH:

8.2 to 8.4

Dissolved oxygen:

7.2 - 8.4

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

300 and 1000 mg/l

Details on test conditions:

See any other information on materials and methods below.

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

462 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was found at 300 mg/L while all fish in 1000 mg/L dose group were dead after 4 h.

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 of FAT 40278/A was determined to be 462.39 mg/L.

Executive summary:

The acute fish toxicity test was performed according to a methodology similar to OECD Guideline 203 (CIBA method A-11). Zebrafish (Danio rerio) were used in the experiment setup. Two groups of fish were tested at the 300 and 1000 mg/L concentration. No mortality was found at 300 mg/L while all fish in 1000 mg/L dose group were dead after 4 h exposure. Hence, the LC50 of FAT 40278/A in this fish acute toxicity test was determined to be 462.39 mg/L.

Description of key information

The acute fish toxicity test was performed according to a method similar to OECD Guideline 203 (CIBA method A-11). Zebrafish (Danio rerio) were used in the experiment setup. Two groups of fish were tested at the 300 and 1000 mg/L concentration. No mortality was found at 300 mg/L while all fish in 1000 mg/L dose group were died after 4 h exposire. Hence, the LC50 of FAT 40278/A in this fish acute toxicity test was determined to be 462.39 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

462.39 mg/L