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EC number: 426-730-3 | CAS number: 123439-82-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant, guideline study, available as unpublished report, fully adequate for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 123439-82-7
- Cas Number:
- 123439-82-7
- IUPAC Name:
- 123439-82-7
- Reference substance name:
- Tetrammine platinum (II) hydrogen carbonate
- IUPAC Name:
- Tetrammine platinum (II) hydrogen carbonate
- Test material form:
- solid
- Remarks:
- powder
- Details on test material:
- - Description: White powder
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration and stability of the test material in the test solutions were verified by chemical analysis at 0 and 48 hours.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- An amount of test material (200 mg) was dispersed in reconstituted water with the aid of ultrasonication and the volume was adjusted to 2 L to give the 100 mg/L test concentration. Dilutions were then made to give the remaining test concentrations: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 mg test item/L.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Daphnia magna were mantained in a laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquee (IRCHA), France
- Age at study initiation: first instar
- Method of breeding: Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated 24 hours prior to the initiation of the test, the young daphnids produced overnight were then removed for testing.
- Feeding during test: none
ACCLIMATION
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: suspension of mixed algae (predominantly Chlorella ssp.)
- Feeding frequency: daily
- Health during acclimation (any mortality observed): none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 270 mg/L as CaCO3
- Test temperature:
- 21 °C
- pH:
- 8.0 - 8.1
- Dissolved oxygen:
- 7.7 - 8.2 mg/l
- Nominal and measured concentrations:
- Nominal concentrations: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg test item/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass jars, containing 200 mL test solution
- Type: closed
- Aeration: No areation
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water, prepared from four stock solutions: a) CaCl2.2H2O 11.76 g/L, b) MgSO4.7H2O 4.93 g/L, c) NaHCO3 2.59 g/L, d) KCl 0.23 g/L. Twenty-five mL of each stock solution a-d were added to each L (final volume) of deionised water. The pH was equal to 7.8 ± 0.2, and adjusted (if necessary) with NaOH or HCl. The reconstituted water was aerated until the dissolved oxygen concentration was approximately air-saturation value.
- Conductivity: < 5 μS/cm
- Total Hardness: 270 mg/L as CaCO3
OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8 hours of dark
EFFECT PARAMETERS MEASURED: any immobilisation or adverse reactions to exposure were recorded at 24 and 48 hours. Daphnids were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.
TEST CONCENTRATIONS
A range-finding study was initially performed at 0.1, 1, 10 and 100 mg/L test concentrations. The results showed no immobilisation at the test concentrations of 0.1, 1 and 10 mg test item/L. However, immobilisation was observed at 100 mg test item/L. Based on this information test concentrations of 1, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg test item/L were selected for the definitive study. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% Confidence Interval: 46 - 59 mg test item/L
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 26.33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% Confidence Interval: 23.3 - 29.9 mg Pt/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% Confidence Interval: 14 - 17 mg test item/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% Confidence Interval: 7.09 - 8.61 mg Pt/L
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 16.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.06 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- Immobilisation data was evaluated using the moving average method of Thompson (1947).
Any other information on results incl. tables
Table 1. Cumulative immobilisation data at 24 and 48 hours of exposure of tetrammineplatinum (2 +) hydrogen carbonate to Daphnia magna.
Nominal concentration (mg test item/L) | Cumulative immobilised Daphnia (Initial population: 10 per replicate) | |||||||
24 hours | 48 hours | |||||||
Replicate 1 | Replicate 2 | Total | % | Replicate 1 | Replicate 2 | Total | % | |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
5.60 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
18 | 0 | 0 | 0 | 0 | 8 | 7 | 15 | 75 |
32 | 0 | 0 | 0 | 0 | 10 | 10 | 20 | 100 |
56 | 7 | 6 | 13 | 65 | 10 | 10 | 20 | 100 |
100 | 10 | 10 | 20 | 100 | 10 | 10 | 20 | 100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, the 48-hour EC50 of tetraammineplatinum (2+) hydrogen carbonate to Daphnia magna was determined to be 15 mg test item/L corresponding to 7.6 mg Pt/L).
- Executive summary:
A 48-h acute immobilisation Daphnia magna study is available for tetraammineplatinum(2+) hydrogencarbonate. The study is reliable without restrictions, being GLP-compliant, and having followed standard test guidelines (OECD 202 and EU Method C.2).
A static test system was followed. Based on a range-finding test, 9 test concentrations were used, and these were analysed using HPLC. Measured test concentrations were closer to nominal with the exception of the lowest test concentration, whose measured values were of 144 % and 194 % of nominal at 0 and 48 hours, respectively. Since this test group was below the estimated NOEC of 10 mg test item/L, results were based on nominal test item concentrations. The 48-hour EC50 was estimated to be 15 mg test item/L corresponding to 7.6 mg Pt/L. The 48-hour NOEC was determined to be 10 mg test item/L (5.06 mg Pt/L).
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