magnesium sodium fluoride silicate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20111-08-15 to 2011-08-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

2006-03-23, Annex 5 corrected 2011-07-28

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

442-650-1

EC Name:

-

Cas Number:

56450-90-9

Molecular formula:

F4Mg5Na2O20Si8

IUPAC Name:

silanedione hydrofluoride magnesium dihydride sodium hydride

Test material form:

solid: particulate/powder

Details on test material:

One sample of Sodium Magnesium Fluoride Silicate, Lot No. 91107
EINECS# 442-650-1
CAS# 56450-90-9
Sample submission date: 16.11.2009

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Topy Industries / 91108
- Expiration date of the lot/batch: 2014-11-20

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: soluble

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: 100mg/L

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

The algal cell count in each flask was measured daily during the test period.
The cell counts of the test solutions were performed by membrane filtration followed by Epifluorescence Microscopy.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: solution
- Controls: yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata. Name in report: Selenastrum capricornutum
- Strain: CCAP 278/4
- Age of inoculum (at test initiation): 3 d
- Method of cultivation: An inoculum culture in the culture medium was prepared 3 days before the start of the test using the same test conditions in order to adapt the test alga to the test conditions and ensure that the algae were in the exponential growth phase when used to inoculate the test substances, positive and negative controls.

ACCLIMATION
- Culturing media and conditions (same as test or not): same
- Abnormal cells: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

Microscopic observation immediately at end of test.

Test conditions

Test temperature:

24 °C +/- 2°C

pH:

5.57 to 6.58 at day 2

Nominal and measured concentrations:

100 mg/L nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: flask
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass
- Renewal rate of test solution (frequency/flow rate): no
- Initial cells density: 1000 - 10000 cells per mL
- Control end cells density: yes
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used: See "Any other information on materials and methods incl. tables."


TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continous lighting
- Light intensity and quality: Continuous cool-white fluorescent lighting with an average light intensity of 6000 – 8880 Lux at the level of the test solutions

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none
- Unusual cell shape: no
- Colour differences: no
- Flocculation: no
- Adherence to test vessels: no
- Aggregation of algal cells: no
- Other: none
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Results with reference substance (positive control):

EC50
- Results with reference substance valid? yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance magnesium sodium fluoride silicate did not exhibit aquatic toxicity to algae in a test according to OECD 201.

Executive summary:

The short-term toxicity study on algae for magnesium sodium fluoride silicate EC# 442-650-1, CAS# 56450-90-9 was determined in a limit test according to OECD 201. No toxicity occured in the limit test with 100 mg/L.

The substance magnesium sodium fluoride silicate did not exhibit aquatic toxicity to algae in a test according to OECD 202.