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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Dermal absorption

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Administrative data

Endpoint:
dermal absorption in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Skin absorption of the industrial catalyst dimethylethylamine in vitro in guinea pig and human skin, and of gaseous dimethylethylamine in human volunteers
Author:
Lundh T, Bowan A, Akesson B
Year:
1997
Bibliographic source:
Int. arch Occup Environ Health 70, 309-313

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Skin absorption of dimethylethylamine in vitro was determined with human and guinea pig skin samples.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyldimethylamine
EC Number:
209-940-8
EC Name:
Ethyldimethylamine
Cas Number:
598-56-1
Molecular formula:
C4H11N
IUPAC Name:
ethyldimethylamine
Details on test material:
no data
Radiolabelling:
no

Test animals

Species:
guinea pig
Strain:
other: albino
Details on test animals or test system and environmental conditions:
Fresh full-thickness albino guinea-pig skin was used (mean 640 g, obtained from Sahlin's Forsoksdjursfarm, Malmo, Sweden). The skin was mounted in Teflon flow-through cells (Vangard International, Neptune, N.J.; Bronaugh 1991). Before exposure, the skin was left to acclimatize for 1 h.

Administration / exposure

Type of coverage:
open
Vehicle:
other: water or isotonic saline solution
Doses:
100 µl of 1% solution (0.67mg/ml)
Control animals:
no

Results and discussion

Signs and symptoms of toxicity:
not examined
Dermal irritation:
not examined
Absorption in different matrices:
DMEA penetrated guinea pig skin.
The median Jss and Kp were 0.009 mg/cm2 x h and 0.001 cm/h, respectively.
No DMEAO could be found in the perfusion medium.
Percutaneous absorption
Dose:
1%
Remarks on result:
other: Kp= 0.001cm/h for guinea pig skin

Any other information on results incl. tables

The steady-state flux permeability coefficient (Kp) and the lag-time obtained in the in vitro experiments.
The respective medians from each experiment are based on data obtained from six flow-through cells
  Experiment n° Jss (mg/cm² x h) Kp (cm/h) Lag-time (h)
Mean Range Mean Range Mean Range
Guinea-pig skin 1 0.008 (0.008-0.011) 0.001 (0.001-0.002) 7 (3 9)
2 0.005 (0.004-0.009) 0.001 (0.001-0.001) Neg b  
3 a 0.025 (0.018-0.027) 0.004 (0.002-0.004) 3 11-5)
4 a 0.009 (0.006-0.023) 0.002 (0.001-0.003) 8 (6-10)
a DMEA diluted with isotonic saline solution
b Negative, the curve shape did not allow lag-time calculation

Applicant's summary and conclusion

Executive summary:

The aims of the study was to assess the skin uptake of dimethylethylamine (DMEA) in vitro from water solutions by fresh guinea-pig specimens. Specimens were analysed by gas chromatography using a nitrogen-sensitive detector. DMEA, diluted with water or isotonic saline solution was applied to fresh guinea-pig skin, mounted in Teflon flow-through cells with a perfusion fluid flow rate of 1.5 ml/h, samples being collected at 2-h intervals for 48 h. DMEA penetrated guinea-pig . The median steady-state flux and permeability coefficient (Kp) values, were 0.009 mg/cm2 x h and 0.001 cm/h, respectively.