Rhodium acetate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 March 1986 – 1 April 1986

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted according to OECD guideline No. 402 and to GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.3 (Acute Toxicity (Dermal))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Rhodium(3+) acetate

EC Number:

247-460-0

EC Name:

Rhodium(3+) acetate

Cas Number:

42204-14-8

Molecular formula:

C6H9O6Rh

IUPAC Name:

Rhodium(3+);triacetate

Test material form:

solid: crystalline

Details on test material:

- Name of test material (as cited in study report): rhodium (III) acetat “braun”
- Substance type: brown crystals
- Physical state: solid
- Composition of test material, percentage of components: 39.54% Rhodium
- Lot/batch No.: 6091
- Stability under test conditions: stable throughout the test period
- Storage condition of test material: at room temperature
- Other: soluble in water

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Savo Ivanovas med., Versuchstierzuchten GmbH, D-7940 Kisslegg in Allgäu
- Age at study initiation: males 3-4 months, females 3 months
- Weight at study initiation: males 2.5-2.65 kg, females 2.25-2.5 kg
- Fasting period before study: food withdrawn on day of treatment
- Housing: individually in wire-bottomed cages
- Diet: ad libitum standard diet, ssniff K “special diet for rabbits” supplied by ssniff Spezialfutter GmbH, D-4770 Soest
- Water: ad libitum
- Acclimation period: at least 5 days before application of test material

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22.5-23.5
- Humidity (%): 45-60
- Photoperiod (hrs dark / hrs light): 6 am-6 pm fluorescent tube lighting, 6 pm-6 am “natural light-dark-rhythm”

Administration / exposure

Type of coverage:

occlusive

Vehicle:

water

Details on dermal exposure:

TEST SITE
- Area of exposure: between the shoulder and the hind leg region
- % coverage: no data
- Type of wrap if used: no data

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: 24 hrs

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg bw
- Constant volume or concentration used: yes
- For solids, paste formed: yes

VEHICLE
- Amount(s) applied (volume or weight with unit): used to dampen the test material

Duration of exposure:

24 hrs

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

3/sex/dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 21 days
- Frequency of observations and weighing: observed 6-8 hours after dosing then twice daily for 21 days or daily on Saturdays, Sundays and public holidays; body weight measured once/week
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No deaths

Clinical signs:

other: No signs of clinical toxicity seen in either the male or female rabbits.

Gross pathology:

No findings.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute dermal median lethal dose (LD50) of rhodium (III) acetate “brown” was found to be >2000 mg/kg bw in rabbits.

Executive summary:

In an OECD Test Guideline 402 study, conducted according to GLP, the acute dermal toxicity of rhodium (III) acetate “brown” was investigated in New Zealand rabbits received. Animals (3/sex) received a single occlusive skin application of the test item (in water) at a limit dose of 2 g/kg bw. After 24 hours, the dressing was removed and the skin washed. The animals were observed twice daily and any deaths were recorded.

 

No mortality was observed at the limit dose, following the 21-day observation period. Slight transient weight loss was apparent in a single female. The dermal LD50 value was determined to exceed 2000 mg/kg bw.

 

Based on the results of this study, no classification is required for acute dermal toxicity according to EU CLP criteria (EC 1272/2008).