N-[3-(diethylamino)phenyl]acetamide

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

Using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, the short term toxicity on aquatic invertebrate was predicted for N-[3-(diethylamino)phenyl]acetamide (Cas no. 6375-46-8). Effect concentration i.e EC50 value was estimated to be 151.18 mg/l for Daphnia magna for 48 hrs duration. Based on the effect value, N-[3-(diethylamino)phenyl]acetamide (Cas no. 6375-46-8) was non toxic to aquatic invertebrates, hence it can be considered to be not classified  as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance N-[3-(diethylamino)phenyl]acetamide . EC50 value was estimated to be 440.58 mg/l for Pseudokirchneriella subcapitata for 72 h duration. This effect value indicating that the substance N-[3-(diethylamino)phenyl]acetamide (CAS no. 6375-46-8) does not pose a significant hazard to algae and thus consider to be not classifies as per the CLP classification criteria.

Additional information

Short-term toxicity to aquatic invertebrates

Five studies including predicted data from validated tools and experimental data from peer reviewed journal and authorative database for short term aquatic invertebrate’s endpoint of test chemical N-[3-(diethylamino)phenyl]acetamide (Cas no. 6375-46-8) with relevant read across which is close to target using log Kow as primary descriptor were summarised as follows:

 

Using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, which indicate the short term toxicity on aquatic invertebrates was predicted for N-[3-(diethylamino)phenyl]acetamide (Cas no. 6375-46-8) and the Effect concentration i.e EC50 value was estimated to be 151.18 mg/l for Daphnia magna for 48 hrs duration.

 

Above predicted result of target assist by experimental result of peer reviewed journal Ecotoxicology 21(7): 2031-2050 for read across chemical Erythromycin (Cas no. 114-07-8) indicate In acute toxicity to aquatic invertebrates species Moina macrocopa (Water Flea) the effective concentrations (EC50) of erythromycin was estimated using probit model. Experiment performs in static fresh water with 25 deg.Test temp. and Moderately Hard condition. Experimental result suggests the effect concentration (EC50) at 48 hrs exposure period for chemical Erythromycin (Cas no. 114-07-8) was observed to be 135.52 mg/l with confidence limit 116.01 - 155.02.

And from ECOTOX database for another read across Methyl 4-acetamido-2-ethoxybenzoate (Cas no. 59-06-3) indicate Short term toxicity to aquatic invertebrates was performed in Daphnia magna (Water Flea) for 48 hrs with <1 d age.Test in fresh water media with static condition. During the experiment, the EC50 value for Methyl 4-acetamido-2-ethoxybenzoate i.e Ethopabate (Cas no. 59 -06 -3) was determined to be 170 mg/l on the basis of mobility.

Same data source (ECOTOX database) for another read across chemical methyl (2S)-2-[N-(2,6-dimethylphenyl)-2-methoxyacetamido]propanoate (Cas no. 57837-19-1) indicate Short term toxicity to aquatic invertebrates was performed in Daphnia magna (Water Flea) for 48 hrs with <1 d age.Test in fresh water media with static condition. During the experiment, the EC50 value for methyl (2S)-2-[N-(2,6-dimethylphenyl)-2-methoxyacetamido]propanoate (Cas no. 57837-19-1) was determined to be 121 mg/l on the basis of mobility.

Last study also from same data source (ECOTOX database) for read across Methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)-DL-alaninate (Cas no. 70630-17-0) also assist all above aquatic effect range such as short term toxicity to aquatic invertebrates was performed in Daphnia magna (Water Flea) for 48 hrs with <=24 h (Neonate)age. Test in fresh water media with static condition. During the experiment, the EC50 value for Methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)-DL-alaninate (Cas no. 70630-17-0) was determined to be 128.26 mg/l on the basis of Mortality.

Thus based on the all available effect concentrations which is in the range of 135.52 to 170 mg/l for target and read across chemicals give the conclusion that test substance N-[3-(diethylamino)phenyl]acetamide (Cas no. 6375-46-8) was likely to be non toxic to aquatic invertebrates as it not meet the classification criteria, hence it can be considered to be not classified as per the CLP regulation.

Toxicity to aquatic algae and cyanobacteria

Four studies including predicted data from validated tool and experimental data from authorative database for toxicity to aquatic algae endpoint of test chemical N-[3-(diethylamino)phenyl]acetamide (Cas no. 6375-46-8) with relevant read across which is close to target using log Kow as primary descriptor were summarised as follows:

 

Using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, which indicate the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance N-[3-(diethylamino)phenyl]acetamide (Cas no. 6375-46-8) .The EC50 value was estimated to be 440.58 mg/l for Pseudokirchneriella subcapitata for 72 h duration. 

 

Above predicted result for target assist by experimental data of read across chemical Caprolactam (Cas no. 105-60-2) from authorative database Japan chemicals collaborative knowledge database (J-check), 2017; indicate toxicity to green algae study was carried out according to OECD Guideline 201 (Alga, Growth Inhibition Test) .The study was based on the growth rate effects of the test compound Caprolactam (Cas no.105-60-2) on green algae. Based on growth effects the EC50 value was observed to be >1000 mg/l and no observed effect concentration (NOEC) was observed to be 1000 mg/l.

 

Another read across Dodecane-12-lactam (Cas no. 947-04-6) from same data source i.e (J-check), 2017 suggest Toxicity to green algae study was carried out .The study was based on the growth rate effects of the test compound Dodecane-12-lactam (Cas no.947-04-6)on green algae. Based on growth effects the EC50 value was observed to be >100 mg/l and no observed effect concentration (NOEC) was observed to be 16 mg/l.

And read across 1-ethenylpyrrolidin-2-one (Cas no. 88-12-0) indicate According to experimental method the 50 percent inhibition of reproduction (EC50) and 50 percent reduction in inhibition of growth rate of chemical 1-ethenylpyrrolidin-2-one in test species Scenedesmus subspicatus was observed to be 770 mg/L and >1000 mg/L, respectively. (OECD HPV Chemical Programme, 2010)

Thus based on the various effects from all above studies give the conclusion that test substance N-[3-(diethylamino)phenyl]acetamide (Cas no. 6375-46-8) was likely to be non toxic to aquatic algae as it not meet the classification criteria of aquatic toxicity as per the CLP regulation.