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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable well documented publication which meets basic scientific principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
yes
Remarks:
observation period: 7 days
Principles of method if other than guideline:
BASF-Test, see details in remarks on material and methods.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): n-Propylamin
- Analytical purity: > 99.5 %

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Analytical verification of test atmosphere concentrations:
yes
Remarks:
Gas chromatography
Duration of exposure:
4 h
Concentrations:
12.0, 7.27, 4.6, 2.84 mg/l analytical value (nominal: 15.9, 14.2, 7.8, 6.4 mg/l)
No. of animals per sex per dose:
10
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Frequency of weighing: before start of the study, on days 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC50
Effect level:
7.06 mg/L air
Exp. duration:
4 h
Sex:
male
Dose descriptor:
LC50
Effect level:
6.32 mg/L air
Exp. duration:
4 h
Sex:
female
Dose descriptor:
LC50
Effect level:
7.87 mg/L air
Exp. duration:
4 h
Mortality:
12 mg/l: 4 males and 8 females died within 24 h, 2 males and 2 females within 48h, 2 males within 72h, 1 male within 4 days and 1 male on day 9.
7.27 mg/l: 3 males and 2 females died within 24 h, 1 male within 48 h and 1 male on day 7.
Clinical signs:
Eye lids closed, watery to reddish eye and nose secretion, salivation, wiping of the snouts, dyspnoea, swollen eyes, nebula, corrosions at nose and eyes, clouded cornea, apathy, crouched position, rough and sticky coat, tremor and staggering walk, bad general condition.

Body weight:
Initially weight loss at 12 mg/l, 7.27 mg/l and 4.6 mg/l.
Gross pathology:
Animals that died:
Heart: acute dilatation (right), acute congestion;
Lung: infarctoic congestion partially accompanied by lung edema, effusion of blood in the thorax; trachea: partially mucosal bleeding in the cranial region,
Swelling of the anogenital region (except rectum), cachexia

Sacrificed animals: no abnormalities.

Any other information on results incl. tables

Mortality:

 Concentration (mg/l)  male  female  
 12.0  10/10  10/10  
 7.27  5/10  2/10  
 4.6  3/10  1/10  
2.84  0/10

 0/10

 

Weight:

Dose (mg/l) Gender  0 h 7 days  14 days
12.0 male 183 118 -
  female 176 - -
 7.27 male  191 182  219
  female 180 160 176
 4.6 male 190 183 221  
  female 188   170 191 
 2.84 male 182 201  237 
  female  178  182 205  
 control male  179  233  278  
  female  175  199  210  

The test substance caused systemic toxicity (including mortality) and local irritation in a dose dependent manner.

Applicant's summary and conclusion