Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report Date:
1986

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
not specified
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: mikrodispers
Details on test material:
Cibanon Blau 83962 Mikrodispers

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
activated sludge

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
No data

Test conditions

Hardness:
No data
Test temperature:
No data
pH:
No data
Dissolved oxygen:
No data
Salinity:
Not applicable
Nominal and measured concentrations:
- Nominal concentrations: 10, 32 and 100 mg/L
Details on test conditions:
No data
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Results with reference substance (positive control):
IC 50 (3 hours) = 21 mg/L
Reported statistics and error estimates:
No data

Any other information on results incl. tables

Results test item:

      3 hours
  Test item in mg/L  O2 -consumption (mg/Liter/min)  Inhibition (%)
 100  1.0  0.0
 32  1.0  0.0
 10  1.0  0.0
 Control 1  0.83  
 Control 2  0.89  

Results reference substance:

    3 hours
   3,5 -Dichlorophenol (mg/L)   O2 -consumption (mg/Liter/min)    Inhibition (%)
 100  0.06  93.1
 32  0.21  75.6
 10  1.03  0

Applicant's summary and conclusion

Conclusions:
IC50 (3 hours ) of the test item is found to be greater than 100 mg/L.
Executive summary:

Introduction

A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2010) No. 209 "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)".

 

 

Methods…….

Activated sewage sludge was exposed to an aqueous solution of the test item at concentrations of 10, 32 and 100 mg/L for a period of 3 hours.

 

The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-dichlorophenol.

 

 

Results…….

The effect of the test item on the respiration of activated sewage sludge gave a 3‑hour EC50value of greater than 100 mg/L. 

 The reference item gave a 3-hour EC50value of 21 mg/L.

Conclusion

The effect of the test item on the respiration of activated sewage sludge gave a 3‑hour EC50value of greater than 100 mg/L. 

 The reference item gave a 3-hour EC50value of 21 mg/L.