Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 250-961-7 | CAS number: 32220-82-9
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Jan to March 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not specified
- GLP compliance:
- no
Test material
- Reference substance name:
- 1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]
- EC Number:
- 250-961-7
- EC Name:
- 1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]
- Cas Number:
- 32220-82-9
- Molecular formula:
- C41H27N7O6
- IUPAC Name:
- 1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bis[3-acetyl-4-aminoanthraquinone]
- Test material form:
- other: mikrodispers
- Details on test material:
- Cibanon Blau 83962 Mikrodispers
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Zebrafish: Danio rerio
Replicates: 10 per test concentration
Age: about 4-6 months
Size:3 cm
Weight: 0.25-0.35 g
Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg.
Further standard conditions:
- Basins have been cleaned with RBS-35 solution (FLUKA 83462) and rinsed with clean water.
- Testing water should be kept at a pH between 6 and 8.5. In case of deviations, pH needs to be adjusted with NaOH or HCl solution.
- Acclimatization period: minimum 12 days at 21-25 °C.
- Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
- Day-night cycles: 12/12h
- Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1% (Methacain).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Not available
- Test temperature:
- 23°C
- pH:
- 7.5 to 7.7
- Dissolved oxygen:
- 7.7 to 8.0
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 100, 300 and 1000 mg/L
- Details on test conditions:
- Water:
- 3 liters for Zebra fish (active coal filtered), Water used according to ISO guidelines.
Basins:
- Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 589.49 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 462.39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - At 100 and 300 mg/L, all fishes survived after 48 and 96 hours of exposure time.
- At 1000 mg/L, all fishes survived until 4 hours of exposure time and all died after 24 hours of exposure time.
Applicant's summary and conclusion
- Conclusions:
- The LC50 of the test substance in this fish acute toxicity test was determined to be 589.49 mg/L and 462.39 after 48 and 96 hours exposure time, respectively.
- Executive summary:
The acute fish toxicity test was performed according to Method A-11.
10 Zebra fish were used in this test at each dose level.
The fish were tested at these differents follwing concentrations: 100, 300 and 1000 mg/L as in the pre-test all fishes survived at 100 mg/L.
- At 100 and 300 mg/L, all fish survived after 48 and 96 hours of exposure time.
- At 1000 mg/L, all fish survived until 4 hours of exposure time and all died after 24 hours of exposure time.
Results:
After 48 hours exposure time:
LC0 =300 mg/L
LC50 = 589.49 mg/L
LC100 =1000 mg/L
After 96 hours exposure time:
LC0 =300 mg/L
LC50 462.39 mg/L
LC100 =1000 mg/L
The LC50 of Vat Blue 66 in this fish acute toxicity test was determined to be 589.49 mg/L and 462.39 mg/L after 48 hours and 96 hours exposure time, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
