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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21 Sep 1999 - 23 Sep 1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
only three concentrations tested, analytical measuremens based on TOC only
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: control and all test concentrations at test start and after 48 h.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 120 mg/L was prepared by weighing the test substance into water. The solution was treated for 60 sec with an ultra-turrax at 8000 rpm and stirred for 1 h on a magnetic stirrer.
- Eluate: no
- Differential loading: no
- Controls: yes, M4-medium
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): < 24 h

ACCLIMATION
- Acclimation conditions (same as test or not): same as test
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
15.4 °dH
Test temperature:
20.0 - 20.3 °C
pH:
8.2
Dissolved oxygen:
5.8 - 5.9 mg/L
Nominal and measured concentrations:
nominal: control, 25, 50, 100 mg/L
measured after 0 h: < 2, 20, 40, 79 mg/L
measured after 48 h: < 2, 21, 40, 77 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: cylindrical test vessels (diameter 4.0 cm, height 6.4 cm)
- Fill volume: 20 mL
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline (M4 medium)
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mobility abter 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
26.7 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
52 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: reported as geom. mean of EC0/EC100
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
101.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: 0%

Table 1: Immobilisation of Daphnia magna after 24 and 48 h

Nominal test substance concentration [mg/L]

Immobile daphnids (absolute)

Immobile daphnids [%]

24 h

48 h

24 h

48 h

Control

0

0

0

0

25

0

0

0

0

50

0

4

0

20

100

16

20

80

100

The test results refer to the concentrations calculated from the measured TOC values. According to the relevant product information, 1 mg/L TOC equals to 1.3 mg/L of the test substance.

Validity criteria fulfilled:
not applicable
Conclusions:
This study resulted in an EC50 (48 h) of 52 mg/L based on mobility of D. magna (EU method C.2, GLP).

Description of key information

EC50 (48 h): 52 mg/L based on immobilisation of D. magna (EU Method C.2).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
52 mg/L

Additional information

One experimental study is available investigating the short-term effects of the substance to aquatic invertebrates. The study was performed according to the EU Method C.2 (GLP) with the water flea Daphnia magna. Nominal concentrations of 25, 50 and 100 mg/L were tested under static conditions. Test concentrations were verified by TOC-analysis at the beginning and end of the exposure period. The measured concentration dropped slightly below 80% at the highest test concentration (77% after 48 h at 100 mg/L). Immobilisation of D. magna was observed after 48 h resulting in an EC50 (48 h) of 52 mg/L based on the geometric mean of measured concentrations.