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EC number: 271-282-2 | CAS number: 68527-77-5
Skin corrosion: No corrosive effects are expected based structural grounds and the 100% application in the LLNA test, not showing corrosive effects.
Skin irritation (OECD TG 439): Irritating Eye irritation and severe damage (OECD TG 405): Irritating
The substance is not considered corrosive because no corrosive functional groups are present: the substance is an alkyl alcohol. The methyl group attached to the double bond is not a corrosive alert either. In addition, no corrosive effects were seen in the LLNA test up to 100%.
In vitro skin irritation test:
The possible skin irritation potential of the substance was tested in vitro using a human skin model through topical application for 15 minutes. The study procedures described in this report were according to OECD TG 439 guideline and GLP principles. Skin tissue was treated by topical application of 25 μL undiluted test substance. After 42 hours incubation period, determination of the cytotoxic (irritancy) effect was performed. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) MTT at the end of treatment. Skin irritation is expressed as the remaining cell viability after exposure to the test substance. Reliable negative and positive controls were included. The positive control had a mean cell viability of 19% after 15 minutes exposure. The standard deviation value of the percentage viability of three tissues treated identically was less than 9%, indicating that the test system functioned properly. The relative mean tissue viability obtained after 15 minutes treatment with the substance compared to the negative control tissue was 12%. Since the mean relative tissue viability for Isocyclogeraniol was below 50% after 15 minutes treatment the substance is considered to be irritant and the substance shall be classified as a skinirritant.
Eye irritation OECD TG 405:
The substance was tested in an eye irritation test in rabbits according to OECD TG 405 test guideline and in compliance with GLP principles. The eyes of three rabbits were exposed to 0.1 mL of the test substance and observations were made up to 14 days after installation. All three animals showed conjunctivae redness ≥2 and in 2 of 3 animals conjunctivae oedema (chemosis) ≥2. Iridial inflammation (score = 1) was noted in two animals. All animals showed opacity ≥1. Observed effects were reversible in all animals within 7 to 14 days except for opacity effects in one animal (score 3 and circumcorneal vascularisation on day 14). Based on the test results, the substance is classified “irreversible effects on the eye.
Based on the positive result in the skin irritation test with the substance and the positive result in the eye irritation test with the read-across substance, the substance needs to be classified as a skin and eye irritant. According to EU Classification, Labeling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008 and GHS this results in skin irritation category 2, H315: Causes skin irritation and serious eye damage category 1, H318: Causes serious eye damage.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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