Phenethyl propionate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed journal.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Acute toxicity studies with insect attractants

Author:

Morton Berozaa, M.N. Inscoea, , P.H. Schwartz Jr.a, M.L. Keplingera, C.W. Mastria

Year:

1975

Bibliographic source:

Toxicology and Applied Pharmacology, Volume 31, Issue 3, March 1975, Pages 421–429

Reference 2

Reference Type:

publication

Title:

A toxicological and dermatological assessment of aryl alkyl alcohol simple acid ester derivatives when used as fragrance ingredients

Author:

D. Belsito , D. Bickers , M. Bruze , P. Calow , M.L. Dagli , A.D. Fryer ,H. Greim , Y. Miyachi , J.H. Saurat , I.G. Sipes

Year:

2012

Bibliographic source:

Food and Chemical Toxicology, 50 (2012) S269–S313

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

An ocular irritation study was conducted for 2-phenylethyl propanoate (CAS No: 122-70-3) on six New Zealand albino rabbits to assess its adverse effects on eye.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Phenethyl propionate

EC Number:

204-567-7

EC Name:

Phenethyl propionate

Cas Number:

122-70-3

Molecular formula:

C11H14O2

IUPAC Name:

2-phenylethyl propanoate

Test material form:

liquid

Details on test material:

- Name of test material : 2-phenylethyl propanoate
- Molecular formula: C11H14O2
- Molecular weight: 178.2296 g/mol
- Smiles notation: c1(CCOC(=O)CC)ccccc1
- InChl: 1S/C11H14O2/c1-2-11(12)13-9-8-10-6-4-3-5-7-10/h3-7H,2,8-9H2,1H3
- Substance type: Organic
- Physical state: Liquid

Specific details on test material used for the study:

- Name of test material (IUPAC name): 2-phenylethyl propanoate
- Common name: Phenethyl propionate (PEP)
- Molecular formula: C11H14O2
- Molecular weight: 178.2296 g/mol
- Smiles notation: c1(CCOC(=O)CC)ccccc1
- InChl: 1S/C11H14O2/c1-2-11(12)13-9-8-10-6-4-3-5-7-10/h3-7H,2,8-9H2,1H3
- Substance type: Organic
- Physical state: Liquid

Test animals / tissue source

Species:

rabbit

Strain:

other: New Zealand albino

Details on test animals or tissues and environmental conditions:

Weight at study initiation: 1.9-3.1 kg

Test system

Vehicle:

not specified

Controls:

yes

Amount / concentration applied:

concentration applied :70%
Amount : 0.1 ml per dose

Duration of treatment / exposure:

7 days

Observation period (in vivo):

1,24,48, 72 hours and 7 days

Number of animals or in vitro replicates:

6 rabbits

Details on study design:

TEST SITE
- Area of exposure: conjunctival sac of right eye of each animal.

SCORING SYSTEM: According to the grading and scoring system of Draize et al. (1944) in which a zero score indicates no irritation and the maximum score at any one scoring period is 110 (maximum irritation and damage to the cornea, iris, and conjunctiva).

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

7 d

Score:

0

Max. score:

110

Reversibility:

fully reversible within: 3 days

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

Slight ocular lesions were observed but all the effects were reversible within 3 days.

Any other information on results incl. tables

Table 1: Primary Irritation score in rabbits

Time Point	Primary Irritation Score
1 hour	12
24 hour	7.5
48 hours	4.6
72 hours	0
7 days	0

Applicant's summary and conclusion

Interpretation of results:

other: Not irritating

Conclusions:

The mean irritation score of chemical 2-phenylethyl propanoate (CAS No: 122-70-3) was observed to be 0.0 after 72 hours. Therefore chemical 2-phenylethyl propanoate (CAS No: 122-70-3) was considered to be not irritating to the eyes of six New Zealand albino rabbits.

Executive summary:

An ocular irritation study was conducted for 2-phenylethyl propanoate (CAS No: 122-70-3) on six New Zealand albino rabbits to assess its adverse effects on eye.

 

The 0.1ml dose of 70% 2-phenylethyl propanoatewas installed into the conjunctival sac of the right eye of each of six rabbit while the left eye of each animal served as the control.

 

Grading for eye injury was made at 1 , 24, 48 and 72 hr and at 7 days after treatment according to the grading and scoring system of Draize et al. (1944) in which a zero score indicates no irritation and the maximum score at any one scoring period is 110 (maximum irritation and damage to the cornea, iris, and conjunctiva).

 

Slight ocular lesions were observed but all the effects were cleared within 3 days. Hence the chemical 2-phenylethyl propanoate (CAS No: 122-70-3) was considered to be not irritating to the eyes of six New Zealand albino rabbits.