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EC number: 810-292-9 | CAS number: 1072-70-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-04-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to OECD guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 5-methyl-1,3-oxazolidin-2-one
- EC Number:
- 810-292-9
- Cas Number:
- 1072-70-4
- Molecular formula:
- C4H7NO2
- IUPAC Name:
- 5-methyl-1,3-oxazolidin-2-one
- Details on test material:
- - Name of test material (as cited in study report): 5-methyl oxazolidin-2-one
- Physical state: Liquid/colorless, clear
- Analytical purity: 98.0 area-% (GC)
- Lot/batch No.: EWALD-00441
- Expiration date of the lot/batch: 19 Jan 2017
- Stability under test conditions: stable
- Storage condition of test material: Ambient at room temperature
- Water solubility: Completely miscible at room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct addition by initial weight into the test flasks. The test
substance was weighed in the required amounts for the test concentrations directly to the test
vessels.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Age: 1 day
Origin: aeration tank of the wastewater treatment plant of Mannheim, Germany
Collection of the test system: 27 Apr 2015
Arrival in the test facility: 27 Apr 2015
- Preparation of inoculum for exposure: After arrival of the activated sludge suspension in the test facility the suspension was sieved
with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over
night at room temperature. At the next day the sludge suspension was washed once with
drinking water and the suspension was adjusted to 3 g/L Dw.
- Sludge concentration in the test: 1.5 g/L Dw
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
Test conditions
- Test temperature:
- 23.1 – 23.4 °C
- pH:
- 7,4-8,4
- Nominal and measured concentrations:
- 1000, 500, 250, 125, 62.5 mg/L as nominal concentration based on test substance without correction of purity and blank
control. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glas-beakers (nominal volume 1L)
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 500 mL
- Aeration: the incubation was started by aeration of the test vessels with pressured air.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- Oxygen concentration during aeration: > 2 mg/L
- Oxygen concentration immediately before measurement: > 7 mg/L
- Duration of the measurement of oxygen consumption: about 8 - 10 min
- Sludge concentration in the test vessel: 1.5 g/L Dw
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 16 mL/test vessel of 100-fold concentrated OECD medium
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations: 1000, 500, 250, 125, 62.5 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 180 min
- Dose descriptor:
- EC10
- Effect conc.:
- 67 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 42.7/103.6
- Duration:
- 180 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 106.7/192.0
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- 620 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 500.2/770.3
- Duration:
- 180 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- The EC50 of 3,5-dichlorophenol: 9.7 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The value of effect concentrations of EC10 EC20 EC50 were given with an accuracy of 2 significant digits. The degree of inhibition was evaluated by Probit analysis according to Finney. The results in this study are consistent with all validity criteria and the test is valid according to the guidelines used for this study. One deviation from the study plan and the test guidelines was realized: Exceeding the test temperature range of 20 ± 2 °C with 1.4 °C. An influence to the results of this study can be excluded.
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