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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
1,4-Di (D-glycosyl) pipeazine derivatives as Oxyuricidal Agents
Author:
Telis A. Martin, Pauls S. Prickit, Allan G. Wheeler, and Howard R. Williams. Jr.
Year:
1963
Bibliographic source:
Journal of Medicinal Chemistry. Vol.6, Pg. 336

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as below
Principles of method if other than guideline:
Acute oral toxicity study was conducted by using Piperazine citrate
GLP compliance:
not specified
Test type:
other: No data
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): Piperazine citrate - Molecular formula: C12H30N6•Cl2H16O14 - Molecular weight: 642.76 g/mole - Substance type: Organic - Physical state: Solid
Specific details on test material used for the study:
- Name of test material (as cited in study report): Piperazine citrate - Molecular formula: C12H30N6•Cl2H16O14 - Molecular weight: 642.76 g/mole - Substance type: Organic - Physical state: Solid

Test animals

Species:
rat
Strain:
not specified
Sex:
male
Details on test animals and environmental conditions:
No data available

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
other: HCl + H2O
Details on oral exposure:
VEHICLE- Concentration in vehicle: 6000, 9000, 12,000 and 15000 mg/kg- Justification for choice of vehicle: HCl + H2O were used
Doses:
6000, 9000, 12,000 and 15000 mg/kg
No. of animals per sex per dose:
Total: 40 6000 mg/kg: 10 rats9000 mg/kg: 10 rats12000 mg/kg: 10 rats15,000 mg/kg: 10 rats
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 14 days (or other?) 24 hrs and 48 hrs
Statistics:
No data available

Results and discussion

Effect levels
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
11 200 mg/kg bw
Based on:
test mat.
95% CL:
>= 9 825 - <= 12 678
Remarks on result:
other: 50% mortality noted
Mortality:
All the treated mice were died after 24 and 48 hrs at 15000 mg/kg and 5 rats after 24 hr and 6 rats after 48 hr died when treated with 12000 mg/kg.
Clinical signs:
Ataxia, diarrhea and depression waas observed in treated rats.
Body weight:
No data available
Gross pathology:
No data available
Other findings:
No data available

Applicant's summary and conclusion

Interpretation of results:
other: not toxic
Conclusions:
LD50 was considered to be 11,200 (12,678-9,825) mg/kg when rats were exposed to Piperazine citrate orally. Thus based on this value and as per CLP classification criteria the substance does not classify as an acute oral toxicant.
Executive summary:

In acute toxicity study, rats were exposed to Piperazine citrate in the concentration 6000, 9000, 12,000 and 15000 mg/kg orally and were observed for 24 and 48 hours. All the treated rats were died after 24 and 48 hrs at 15000 mg/kg and 5 rats after 24 hr and 6 rats after 48 hr died when treated with 12000 mg/kg and Ataxia, diarrhea and depression was observed in treated rats.Therefore, LD50 was considered to be 11,200 (12,678-9,825) mg/kg when mice were exposed to Piperazine citrate orally.

According to the publication and CLP clasiification criteria the test material does not classify as an acute oral toxicant.