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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
calculation (if not (Q)SAR)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
accepted calculation method
Justification for type of information:
Data is from modeling Danish QSAR database.
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
The prediction is done using Danish (Q)SAR Database
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (as cited in study report): Piperazine citrate / tripiperazine dicitrate- Molecular formula: C12H30N6•Cl2H16O14 - Molecular weight: 642.76 g/mole - Substance type: Organic - Physical state: Solid
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
213.428 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
not specified
Conclusions:
Based on the QSAR prediction done using the Danish (Q)SAR Database, the 48 hours EC50 was estimated to be 213.4281mg/l on Daphnia Magna for substance Piperazine Citrate with immobilization effects. Thus based on this value it can be concluded that the substance can be considered as not classified as per the CLP regulation
Executive summary:

48 hrs aquatic toxicity study (Danish QSAR, 2016) was conducted to assess toxic effects of the Piperazine Citrate(CAS no 144-29-6) and the results were predicted. The study was based on the effects of the test compound on Daphnia magna in a static fresh water system. The predicted data suggests the effective concentration (EC50) for thePiperazine Citrate was estimated to be 213.4281 mg/l. 

On the basis of results for toxicity to aquatic invertebrates, it can be considered that the substance is not likely to be toxic to aquatic invertebrates and can be considered as not classified as per the CLP regulation

Description of key information

EC50 value of piperazine 2-hydroxypropane-1,2,3-tricarboxylate (3:2) (salt) (CAS:144-29-6) was estimated to be 218.80 mg/l for Daphnia magna after 48 hrs of duration, by QSAR toolbox. Thus based on this value it can be concluded that the substance can be considered as not classified as per the CLP regulation

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
218.8 mg/L

Additional information

Predicted data of the test compound Piperazine Citrate and its read-across substance were reviewed from reliable sources for short term toxicity to aquatic invertebrates and are presented below as weight of evidence approach:

In key study, based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic invertebrates was predicted for piperazine 2-hydroxypropane-1,2,3-tricarboxylate (3:2) (salt) (CAS:144-29-6). EC50 value was estimated to be 218.80 mg/l for Daphnia magna after 48 hrs duration. Based on the value, the substance piperazine 2-hydroxypropane-1,2,3-tricarboxylate (3:2) (salt) is considered to be non-toxic to aquatic invertebrates and can be considered to be not classified as per the CLP regulations.

Supporting above prediction, 48 hrs aquatic toxicity study (Danish QSAR, 2016) was conducted to assess toxic effects of thePiperazine Citrate(CAS no 144-29-6) and the results were predicted. The study was based on the effects of the test compound on Daphnia magna in a static fresh water system. The predicted data suggests the effective concentration (EC50) for thePiperazine Citratewas estimated to be 213.4281 mg/l. 

Further, Short term toxicity to aquatic invertebrates test for read across piperazine (110-85-0) (J-check, 2013) was performed according to the OECD guideline 202.The test was conducted for 48 hrs and EC 50 value was observed. After experiment the EC50 value for short term toxicity to aquatic invertebrates for piperazine (110-85-0) was determined to be 110mg/l.

On the basis of results for toxicity to aquatic invertebrates for target and read across substance and by applying weight of evidence approoach, it can be considered that the substance is not likely to be toxic to aquatic invertebrates and can be considered as not classified as per the CLP regulation.