Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: screening test, other
Remarks:
Inherent Biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report Date:
1993

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
not applicable
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
Active su
Specific details on test material used for the study:
HMPPJC, dark brown liquid in original container at room temperature

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
A mixed population of activated sewage sludge microorganism
Duration of test (contact time):
>= 28 d
Initial test substance concentration
Initial conc.:
ca. 108.9 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Reference substance
Reference substance:
diethylene glycol
Remarks:
Digol

Results and discussion

% Degradation
Key result
Parameter:
% degradation (DOC removal)
Value:
ca. 24
Sampling time:
28 d

Applicant's summary and conclusion

Interpretation of results:
not inherently biodegradable
Executive summary:

HMPPJC attained 24% degradation after 28 days and so, therefore, cannot be considered as inherently biodegradable udner the stric terms and conditions of the OECD Guidelines.

The standard material, Digol, attained 99%degradation after 28 days, thereby confirming the suitability of the inoculum and culture conditions.
pF values did not drop below 6.7 in any culture vessel during the course of the study and were adjusted to pH 7.4 daily. Dissolved oxygen levels remained at approximately 6.4 mg O2/l or above in all cultures at all times.