Phenethyl benzoate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

accepted calculation method

Justification for type of information:

Data is from computational model developed by USEPA

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

Long term toxicity to aquatic invertebrate daphnia was carried out according to the EPISUITE.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea

Test type:

not specified

Water media type:

not specified

Total exposure duration:

21 d

Reference substance (positive control):

not specified

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.316 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Other details not known

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of no effects observed in a static freshwater system, the NOEC value for the substance is estimated to be 0.316 mg/l for daphnia for 21 days of exposure duration.

Executive summary:

Based on the prediction done using ECOSAR class, the long term toxicity on aquatic invertebrate daphnia was predicted for test substance. On the basis of no effects observed in a static freshwater system, the NOEC value for the substance is estimated to be 0.316 mg/l for daphnia for 21 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as toxic to aquatic invertebrate daphnia at environmentally relevant concentrations and can be considered to be classified in aquatic chronic 2 category as per the CLP classification criteria. 

Description of key information

Based on the prediction done using ECOSAR class, the long term toxicity on aquatic invertebrate daphnia was predicted for test substance. On the basis of no effects observed in a static freshwater system, the NOEC value for the substance is estimated to be 0.316 mg/l for daphnia for 21 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as toxic to aquatic invertebrate daphnia at environmentally relevant concentrations and can be considered to be classified in aquatic chronic 2 category as per the CLP classification criteria. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.316 mg/L

Additional information

Based on the various long term studies available for the test chemical, studies were reviewed to determine the toxic nature of test chemical on the mobility and reproduction of aquatic invertebrates. The studies are as mentioned below:

 

Based on the prediction done using ECOSAR class, the long term toxicity on aquatic invertebrate daphnia was predicted for test substance. On the basis of no effects observed in a static freshwater system, the NOEC value for the substance is estimated to be 0.316 mg/l for daphnia for 21 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as toxic to aquatic invertebrate daphnia at environmentally relevant concentrations and can be considered to be classified in aquatic chronic 2 category as per the CLP classification criteria. 

 

Above study was supported by the second study. 21 days long term toxicity tests of test chemical were performed on daphnia magna. After the exposure of test chemical with daphnia magna for 21 days, no effects were observed on the reproduction rate of daphnia magna. Thus the NOEC was observed at 0.84 mg/l. Based on the NOEC value, chemical consider to be toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

Similar aim of this study was to access the effect of test chemical on the reproduction and growth rate of aquatic invertebrate daphnia magna. Test conducted for 10 days. Based on the low effect of test chemical on the growth and reproduction of daphnia magna after the exposure of 10 days, LOEC was observed at 0.1 mg/l. Based on the LOEC, chemical consider to be toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

 

In the fourth study long term toxicity test was performed on Daphnia magna for 21 days according to the OECD guideline 211. 20 ml test tubes were used as a vessel with different concentrations in which 1 larva at each test tubes were kept for experiment. After 48 hrs test water were replaced and count the cumulative numbers of broods. Based on that both Immobilized bodies and cumulative numbers of broods NOEC was determined. On the basis of above experiment the NOEC value for test chemical to Daphnia magna was found to be 0.84 mg/l concentration.

Thus based on the above effects and studies available on the long term effect on aquatic invertebrate, test chemical consider to be toxic to the aquatic invertebrates and hence classified in aquatic chronic 2 category as per the CLP classification criteria.

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