4-(2,5,6,6-tetramethylcyclohex-2-enyl)but-3-en-2-one

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 191.1 (f) (1) of the Final Order, Federal Register, Vol. 26, No. 155, P. 7336, 12 August 1961

GLP compliance:

no

Remarks:

study pre-dates introduction of GLP

Test material

Test material information

Constituent 1

Reference substance name:

rel-(E)-4-((1R,5R)-2,5,6,6-tetramethylcyclohex-2-en-1-yl)but-3-en-2-one

Cas Number:

599-45-1

Molecular formula:

C14H22O

IUPAC Name:

rel-(E)-4-((1R,5R)-2,5,6,6-tetramethylcyclohex-2-en-1-yl)but-3-en-2-one

Constituent 2

Reference substance name:

rel-(E)-4-((1R,5S)-2,5,6,6-tetramethylcyclohex-2-en-1-yl)but-3-en-2-one

Cas Number:

472-46-8

Molecular formula:

C14H22O

IUPAC Name:

rel-(E)-4-((1R,5S)-2,5,6,6-tetramethylcyclohex-2-en-1-yl)but-3-en-2-one

impurity 1

Reference substance name:

3-Buten-2-one, 4-(2,5,6,6-tetramethyl-1-cyclohexen-1-yl)-, (3E)-

Cas Number:

72074-84-1

Molecular formula:

C14H22O

IUPAC Name:

3-Buten-2-one, 4-(2,5,6,6-tetramethyl-1-cyclohexen-1-yl)-, (3E)-

impurity 2

Reference substance name:

3-Buten-2-one, 4-(2,2,3,6-tetramethyl-3-cyclohexen-1-yl)-, [1α(E),6α]-

Cas Number:

140632-46-8

Molecular formula:

C14H22O

IUPAC Name:

3-Buten-2-one, 4-(2,2,3,6-tetramethyl-3-cyclohexen-1-yl)-, [1α(E),6α]-

impurity 3

Reference substance name:

4-Penten-2-one, 5-(1,3,4-trimethyl-3-cyclohexen-1-yl)-, (E)-

Cas Number:

162282-94-2

Molecular formula:

C14H22O

IUPAC Name:

4-Penten-2-one, 5-(1,3,4-trimethyl-3-cyclohexen-1-yl)-, (E)-

Specific details on test material used for the study:

RIFM 72-10-26 a-Irone

Test animals

Species:

rat

Strain:

other: Sherman-Wistar

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Doses:

5 mg/kg

No. of animals per sex per dose:

5 animals per sex per dose

Control animals:

not specified

Details on study design:

Two groups, consisting of five male and five female albino rats, were set aside and observed for a period of one week to assure nomalcy. The animals were then starved for 24 hours. Doses were determined for each animal and administered by means of a syringe and stomach tube. Following ths, the animals were allowed food and water ad libitum during a fourteen-day observation period.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

5 000 mg/kg bw

Based on:

test mat.

Other findings:

Symptoms:
Non-survivors: Morbidity soon after dosing followed by prostration, coma and death
Survivors: Lethargy, recovery 24-48 hours

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Irone Alpha, as tested in rats, has an acute oral LD50 of greater than 5.0 gm/kg and is not a toxic substance. The test material does not require cautionary labelling in accordance with 191.1 (f) (1) of the Final Order, Federal Register, Vol. 26, No. 155, P. 7336, 12 August 1961.