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EC number: 216-938-0 | CAS number: 1703-58-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from Cosmetic Ingredient Review.
Data source
Reference
- Reference Type:
- publication
- Title:
- Determination of skin sensitization of Citric acid.
- Author:
- Cosmetic Ingredient Review Expert Panel
- Year:
- 2 012
- Bibliographic source:
- Cosmetic Ingredient Review, 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- To access skin sensitization by Human Repeat Insult Patch Test (HRIPT).
- GLP compliance:
- not specified
- Type of study:
- not specified
Test material
- Reference substance name:
- Citric acid
- EC Number:
- 201-069-1
- EC Name:
- Citric acid
- Cas Number:
- 77-92-9
- Molecular formula:
- C6H8O7
- IUPAC Name:
- citric acid; 3-hydroxy-3-carboxy-1,5-pentanedioic acid
- Test material form:
- solid: crystalline
- Details on test material:
- Name of the test chemical: citric acid; 3-hydroxy-3-carboxy-1,5-pentanedioic acidMolecular Formula:C6H8O7Molecular Weight: 192.122 g/molInChI: 1S/C6H8O7/c7-3(8)1-6(13,5(11)12)2-4(9)10/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12)Substance Type: OrganicPhysical State: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Citric acid- Molecular formula : C6H8O7- Molecular weight : 192.122 g/mol- Substance type: Organic- Physical state: solid
In vivo test system
Test animals
- Species:
- other: Human
- Strain:
- other: not applicable
- Sex:
- not specified
Study design: in vivo (non-LLNA)
Induction
- Route:
- epicutaneous, semiocclusive
- Vehicle:
- other: Cuticle cream
- Concentration / amount:
- 4%
- Day(s)/duration:
- 21 days
- Adequacy of induction:
- not specified
Challenge
- Route:
- epicutaneous, occlusive
- Concentration / amount:
- 4%
- No. of animals per dose:
- 56 humans
- Details on study design:
- MAIN STUDYA. INDUCTION EXPOSURE- No. of exposures: 3- Exposure period: 3 weeks - Test groups: 56 patient- Frequency of applications: 3x/wk - Duration: 3 weeks- Concentrations: 4%B. CHALLENGE EXPOSURE- Exposure period: challenge patch wasapplied after 2 wks
- Positive control substance(s):
- not specified
Results and discussion
In vivo (non-LLNA)
Results
- Reading:
- 1st reading
- Group:
- test chemical
- Dose level:
- 4%
- No. with + reactions:
- 0
- Total no. in group:
- 56
- Clinical observations:
- No sensitization
- Remarks on result:
- no indication of skin sensitisation
Applicant's summary and conclusion
- Interpretation of results:
- other: non-sensitizing
- Conclusions:
- The citric acid (77-92-9) was considered to be non-toxic to human patient and based on that can be considered to be non-sensitizer.
- Executive summary:
Skin sensitization test was performed in 56 patients by applying 4% in a cuticle cream by HRIPT methods. Semi occlusive patches were applied 3x/wk for 3 wks in induction exposure and continue after 2 week in challenge exposure.After the experiment, no reaction was obtain hence, the citric acid was considered to non-toxic to human patient and based on that can be considered to be non-sensitizer.
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