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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short-term toxicity to aquatic invertebrates

The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 2.2 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 2 category as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

The median effective concentration (EC50) for the test substance, in algae was determined to be 4.3 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 2 category as per the CLP classification criteria.

Toxicity to microorganisms

In Advisory Board for Environmentally Relevant Existing Substances (BUA);2000 report the ecotoxicological and toxicological aspect of chemical was studied.According to report the effect concentration (EC0) for bacteria Escherichia coli was observed to be 50 mg/l for 96 hrs exposure period.Based on the effect concentration it may considered that the test chemical may have aquatic toxicity concern to micro organism at higher dose concentrations for acute exposure.

Additional information

Short-term toxicity to aquatic invertebrates

Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilization Test) in a static system for the total exposure period of 48 hrs.

 The stock solution 10 g/l was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changeable sentence according to the chemical).0.5, 1, 2, 4 and 8 mg/l concentrations were used in the study. Effects on immobilization were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 2.2 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 2 category as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100.0 g/l was prepared by dissolving colorless liquid in acetone in OECD growth medium / reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Various concentrations were used.

 

With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determined after an exposure period of 72 hrs.

 

The median effective concentration (EC50) for the test substance, in algae was determined to be 4.3 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 2 category as per the CLP classification criteria.

Toxicity to microorganisms

Two studies including experimental data from peer reviewed journal and authorative database for toxicity to micro organism endpoint of test chemical with relevant read across which is close to target by structure were summarised as follows:

First study of target from secondary source Advisory Board for Environmentally Relevant Existing Substances (BUA) which suggests the ecotoxicological and toxicological aspect of chemical was studied and according to report the effect concentration (EC0) for bacteria Escherichia coli was observed to be 50 mg/l for 96 hrs exposure period.Based on the effect concentration it may considered that the test chemical may have aquatic toxicity concern to micro organism at higher dose concentrations for acute exposure.

Anoher peer reviewed journal indicate the 50 percent growth impairment concentration (IGC 50) of test chemical in the micro-organism Tetrahymena pyriformis during a 48 hr study was reported as 0.67mg/L.The observed growth impairment concentration suggest that the chemical may have concern to toxicity to micro organism for acute exposure period.

Both experimental study of toxicity to micro organism give the conclusion that the test chemical may have toxicity concern for acute exposure.