3-methyl-1-phenylpyrazol-5-ylamine

Endpoint summary

Description of key information

Short term toxicity to fish:

Study was conducted to assess the effect of test chemical 3-methyl-1-phenylpyrazol-5-ylamine on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Limit test were performed at 100 mg/l. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentration 100 mg/l, LC50 was determine to be > 100 mg/l . Based on the LC50, it can be consider that the chemical 3-methyl-1-phenylpyrazol-5-ylamine was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrate:

Aim of this study was to assess the short term toxicity of 3-methyl-1-phenyl-1H-pyrazol-5-amine to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 100 mg/l was prepared by dissolving white powder in reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 5, 10, 20, 40 and 80 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance 3-methyl-1-phenyl-1H-pyrazol-5-amine, in Daphnia magna was determined to be 25.8 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to aquatic algae:

Objective of this study was to observe the action of test chemical when it exposed with the Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) for 72 hrs. Test conducted in accordance with OECD Guideline 201 (Alga, Growth Inhibition Test). The stock solution 160 mg/l was prepared by dissolving white powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. 0, 10, 20, 40, 80 and 160 mg/l concentrations were used. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration ErC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determined after an exposure period of 72 hrs. The median effective concentration (ErC50) for the test substance 3-methyl-1-phenyl-1H-pyrazol-5-amine, in algae was determined to be 100.2 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the ErC50 value, which indicates that the substance is likely to be non-hazardous to aquatic algae and cannot classified as per the CLP classification criteria.

Additional information

Short term toxicity to fish:

Study was conducted to assess the effect of test chemical 3-methyl-1-phenylpyrazol-5-ylamine on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Limit test were performed at 100 mg/l. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentration 100 mg/l, LC50 was determine to be > 100 mg/l . Based on the LC50, it can be consider that the chemical 3-methyl-1-phenylpyrazol-5-ylamine was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrate:

Aim of this study was to assess the short term toxicity of 3-methyl-1-phenyl-1H-pyrazol-5-amine to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 100 mg/l was prepared by dissolving white powder in reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 5, 10, 20, 40 and 80 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance 3-methyl-1-phenyl-1H-pyrazol-5-amine, in Daphnia magna was determined to be 25.8 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to aquatic algae:

Objective of this study was to observe the action of test chemical when it exposed with the Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) for 72 hrs. Test conducted in accordance with OECD Guideline 201 (Alga, Growth Inhibition Test). The stock solution 160 mg/l was prepared by dissolving white powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. 0, 10, 20, 40, 80 and 160 mg/l concentrations were used. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration ErC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determined after an exposure period of 72 hrs. The median effective concentration (ErC50) for the test substance 3-methyl-1-phenyl-1H-pyrazol-5-amine, in algae was determined to be 100.2 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the ErC50 value, which indicates that the substance is likely to be non-hazardous to aquatic algae and cannot classified as per the CLP classification criteria.

Thus based on the toxicity test on aquatic invertebrate daphnia magan, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.