2-Propanol, 1-butoxy-3-(2-propen-1-yloxy)-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 April 2013 to 26 April 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Specific details on test material used for the study:

Physical state/Appearance: Brown Liquid
Batch: 12D25
Purity: 94.0%

Analytical monitoring:

yes

Details on sampling:

Based on the results of the range-finding test, the concentration for the definitive test was set as follows:
Control: n/a
Conc.1*: 110mg/L

*. Limit test at the corrected value by purity which is the maxImum concentration given in the Test Guideline.

Vehicle:

yes

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Medaka
- Source: Breeding in the testing facility
- Age at study initiation: Two year or less after hatching
- Length at study initiation (length definition, mean, range and SD): Measurements: 2.30 cm (1.93 - 2.68 cm), n=10
(These values were measured and weighed for the fish of control at the end of exposure.)
- Weight at study initiation (mean and range, SD): Measurements: 0.101 g (0.054 - 0.153 g), n=10
(These values were measured and weighed for the fish of control at the end of exposure.)
- Feeding during test: None
- Food type:n/a
- Amount: n/a
- Frequency: n/a

ACCLIMATION
- Acclimation period: 2 weeks

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Test fish were observed at 3, 24, 48, 72 and 96 hours after the start of exposure.

Hardness:

The total hardness was 60 mg CaC03/L (within the appropriate range)

Test temperature:

24±1°C

pH:

7.8 (within the appropriate range)

Dissolved oxygen:

80% or more of the saturation (not aerated)

Nominal and measured concentrations:

Measured Concentrations of the Test Substance in Test Solutions

Test Group Nominal conc. Measured concentration (mg/L)
(mg/L) (Percent of Nominal, %)
0hr New 24 hr Old 72hr New 96 hr Old Mean

Control n/a <0.01 <0.01 <0.01 <0.01 n/a

Conc.1 110 107 121 117 122 117
(97) (110) (106) (111) (106)


New: New test solution freshly prepared
Old: Old test solution immediately prior to renewal or at the end of exposure
Mean: Time weighted mean

Details on test conditions:

TEST SYSTEM
- Test vessel: 5-L glass vessel (cover: clear polyvinyl chloride (PVC) plate)
- Number of vessels: 1 vessel/test group
- Type of flow-through (e.g. peristaltic or proportional diluter): Biomass loading: 1 g fish/L/day or less)
- Biomass loading rate: 10 fish/test group

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated water processed by treating Yokohama city tap water· with an activated charcoal filter and sodium thiosulfate. Water quality parameters of the dechlorinated water have been measured periodically (about every 6 months) by Mitsubishi Chemical Analytech Co., Ltd., and confirmed to be appropriate to use for dilution water (total hardness: 10-250 mg CaC03/L, pH: 6.7-8.5).
- Total organic carbon: 0.3mg/L

OTHER TEST CONDITIONS
- Adjustment of pH: Not adjusted
- Photoperiod: 16-hour light / 8-hour dark
- Light intensity: 1000 lux or less


TEST CONCENTRATIONS
- Range finding study: 1.1, 11, 110 mg/L
- Test concentrations: 110mg/L
- Results used to determine the conditions for the definitive study: No mortality for 96 hours, No toxicological symptoms

Reference substance (positive control):

yes

Remarks:

(CuS04(II)·5H2O, guaranteed reagent

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 110 mg/L

Conc. based on:

test mat.

Remarks on result:

other: Effect concentration corrected with purity ( 94%). 95% confidence limits: not available.

Details on results:

- Behavioural abnormalities: 0
- Observations on body length and weight:
- Mortality of control: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:

Sublethal observations / clinical signs:

Median Lethal Concentrations (LC50)

Exposure period(hr)	LC50	95 -Percent confidence limits (mg/L)	Statistical method
24	>117*[>110*]	-	-
48	>117*[>110*]	-	-
72	>117*[>110*]	-	-
96	>117*[>110*]	-	-

*: The LC50 (LL50) value was given as an estimated concentration range because this test

was a limit test.

[]: Corrected with purity

Validity criteria fulfilled:

yes

Conclusions:

Result based on the time-weighted mean of measured concentrations: 96-hour LC50: >117 mg/L
Result corrected by purity: 96-hour LC50: >110 mg/L

Description of key information

96-hour LC50 in Medaka >117 mg/L: Result based on the time-weighted mean of measured concentrations
96-hour LC50 in Medaka >110 mg/L: Result corrected by purity
No mortality at 110 mg/L (Nominal concentration).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

117 mg/L