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EC number: 943-368-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- Chemical analysis was performed in a non-GLP laboratory
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Chemical analysis was performed in a non-GLP laboratory
- Specific details on test material used for the study:
- - Source and lot/batch No. of test material: Rivertop Renewables, lot number 150330EAA
- Expiration date of the lot/batch: March 15, 2017
- Purity test date: Purity – 97.7%%
- Water solubility: 51.2%
- Physical state - Brown liquid - Analytical monitoring:
- yes
- Remarks:
- Chemical analysis was performed in a non-GLP laboratory
- Details on sampling:
- Duplicate samples, approximately 10 to 15 mL from each replicate test or control vessel, were taken for analysis at the start (0 hours) and end (72 hours) of the exposure period. Samples were kept cool after sampling and were transferred to the analytical laboratory within 24 hours of sampling. Samples were analysed within apporximately 24 hours of sample reception.
- Vehicle:
- no
- Details on test solutions:
- All solutions of the test item were corrected for reported water solubility (i.e. 51.2% by weight in water). 781 mg of test material was weighed into a 500 mL volumetric flask and made up to volume with nutrient medium.
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Source (laboratory, culture collection): University of Waterloo Culture Collection, CPCC 37
- Age of inoculum (at test initiation): 3 days and in exponential growth
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same
- Any deformed or abnormal cells observed: none reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- Not applicable
- Test temperature:
- 22°C
- pH:
- Range between 7.36 and 7.93
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Conductivity:
- Not applicable
- Nominal and measured concentrations:
- Nominal exposure concentrations were 0 (control), 6.6, 14.8, 32.8, 72.9, 162, 360 and 800 mg/L.
Time weighted mean concentratinos were 0, less than limit of detection (nominal 6.6 and 14.8), 13, 30, 68, 147 and 333 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlemeyer flasks
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 50 mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: constant
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: haemacytometer
- Reference substance (positive control):
- yes
- Remarks:
- Results are presented for sodium chloride and include 20 historical values.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 169 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 60.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 68 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 333 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 141 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- Results are presented for sodium chloride and include 20 historical values.
- Reported statistics and error estimates:
- ANOVA/Dunnetts Multiple Comparion tests, Non-linear regression, Steel Many-One Rank Sum Test were performed using the CETISTM software
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the reported information an acceptable assessment of toxicity to Pseudokirchneriella subcapitata was achieved. Based on the time weighted arithmetic mean concentrations of D-Glucose, reaction products with nitric acid and sodium nitrite (1:1), sodium salts the following results were obtained:
Biomass – 72 h EyC50– 169 mg/L, 72 h EyC10 – 60.7 mg/L, NOEC 68 mg/L
Growth rate - 72 h ErC50– >333 mg/L, 72 h ErC10 – 141 mg/L, NOEC 30 mg/L - Executive summary:
The effects of D-Glucose, reaction products with nitric acid and sodium nitrite (1:1), sodium salts to the algae Pseudokirchneriella subcapitata were determined in a 72 hour static study. The study was carried out according to OECD guideline 201. In this study algae were exposed to D-Glucose, reaction products with nitric acid and sodium nitrite (1:1), sodium salts over a test period of 72 hours in in a dose response study. Nominal concentrations were 0 (control), 6.6, 14.8, 32.8, 72.9, 162, 360 and 800 mg/L, corresponding time weighted arithmetic mean measured concentrations were 0, less than the limit of detection (6.6 and 14.8 mg/L), 13, 30, 68, 147 and 333mg/L.
Based on the time weighted arithmetic mean concentrations the following results were obtained:
Biomass – 72 h EyC50– 169 mg/L, 72 h EyC10 – 60.7 mg/L, NOEC 68 mg/L Growth rate - 72 h ErC50– >333 mg/L, 72 h ErC10 – 141 mg/L, NOEC 30 mg/L
Reference
Analytical results, 0, 72 hour results and mean, <MDL = <6 mg/L
Control, <MDL, <MDL, na
6.6 mg/L, <MDL, <MDl, na
14.8 mg/L, <MDL, <MDL, na
32.8 mg/L, 14.3, 11.7, 13.0
72.9 mg/L, 34.1, 26.2, 30
162 mg/L, 78.1, 58.3, 68
360 mg/L, 181.9, 117.3, 147
800 mg/L, 400.6, 273.3, 333
Cell density results were as follows for 24, 48 and 72 hours,
Control - 5.25, 27.38, 139.0
6.6 mg/L - 4.38, 27.0, 156.75, inhibition -12.77%
14.8 mg/L - 4.13, 28.88, 152.0, inhibition -9.35%
32.8 mg/L - 3.63, 29.0, 132.38, inhibition - 4.77%
72.9 mg/L - 2.75, 25.25, 157.75, inhibition -13.49%
162 mg/L - 4.0, 25.38, 124.13, inhibition - 10.7%
360mg/L - 3.0, 17.63, 84.63, inhibition - 39.12%
800mg/L - 2.0, 6.63, 15.0, inhibition - 89.21%
Area under the growth curve results, 0 - 72 hours and % inhibition
Control, 1.73, -
6.6mg/L - 1.77, -2.29%
14.8 mg/L - 1.76, -1.68%
32.8 mg/L - 1.71, 0.96%
72.9 mg/L - 1.77, -2.37%
162 mg/L - 1.69, 2.19%
360 mg/L - 1.57, 9.58%
800 mg/L - 0.97, 43.83%
Yield results (x 10000 cells/ml), 72 hours and % inhibition
Control - 138.23, -
6.6 mg/L - 155.98, -12.84%
14.8 mg/L - 151.23, -9.40%
32.8 mg/L - 131.6, 4.79%
72.9 mg/L - 156.98, -13.57%
162 mg/L - 123.35, 10.76%
360 mg/L - 83.85, 39.34%
800 mg/L - 14.24, 89.71%
Description of key information
The effects of D-Glucose, reaction products with nitric acid and sodium nitrite (1:1), sodium salts to the algae Pseudokirchneriella subcapitata were determined in a 72 hour static study. The study was carried out according to OECD guideline 201. In this study algae were exposed to D-Glucose, reaction products with nitric acid and sodium nitrite (1:1), sodium salts over a test period of 72 hours in in a dose response study. Nominal concentrations were 0 (control), 6.6, 14.8, 32.8, 72.9, 162, 360 and 800 mg/L, corresponding time weighted arithmetic mean measured concentrations were 0, less than the limit of detection (6.6 and 14.8 mg/L), 13, 30, 68, 147 and 333mg/L.
Based on the time weighted arithmetic mean concentrations the following results were obtained:
Biomass – 72 h EyC50– 169 mg/L, 72 h EyC10 – 60.7 mg/L, NOEC 68 mg/L Growth rate - 72 h ErC50– >333 mg/L, 72 h ErC10 – 141 mg/L, NOEC 30 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 141 mg/L
- EC10 or NOEC for freshwater algae:
- 30 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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