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EC number: 203-628-5 | CAS number: 108-90-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
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- Stability: thermal, sunlight, metals
- pH
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- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial aspect ratio / shape
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- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD, EU and Japan Guidelines. Non-GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2600 (Skin Sensitisation)
- GLP compliance:
- no
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Chlorobenzene
- EC Number:
- 203-628-5
- EC Name:
- Chlorobenzene
- Cas Number:
- 108-90-7
- Molecular formula:
- C6H5Cl
- IUPAC Name:
- chlorobenzene
- Details on test material:
- - Name of test substance: Chlorobenzene
- Test-substance No.: 08/0386-2
- CAS No.: 108-90-7
- Batch Identification: S90576-359
- Form of application: solution in AOO (4+1, v/v)
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- other: CBA/J
- Sex:
- female
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 100, 50 and 25 %
- No. of animals per dose:
- 5
- Details on study design:
- Three days after the last application the mice were injected into the tail vein with 20 μCi of 3Hthymidine in 250 μL of sterile saline. About 5 hours after the 3H-thymidine injection, the mice were sacrificed and the auricular lymph nodes were removed. Lymph node response was evaluated by measuring 3H-thymidine incorporation (indicator of cell proliferation). Cell counts and weights of each animal’s pooled lymph nodes were also determined.
Additionally, flow cytometric analysis using aliquots of the lymph node cell suspension was performed for determination of lymphocyte subpopulations (immunophenotyping). Moreover, a defined area with a diameter of 0.8 cm was punched out of the apical part of each ear and for each animal the weight of the pooled punches was determined to obtain an indication of possible skin irritation. - Statistics:
- Statistical evaluations were performed using the WILCOXON-test (# for p ≤ 0.05, ## for p ≤ 0.01) for 3H-thymidine incorporation, cell count, lymph node weight and ear weight and KRUSKAL-WALLIS test and WILCOXON test for flow cytometric endpoints.
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: see Remark
- Remarks:
- 3H-thymidine incorporation - vehicle AOO: 1.00 - 25% in AOO: 1.91 - 50% in AOO: 3.23 - undiluted test substance: 5.30 Cell count - vehicle AOO: 1.00 - 25% in AOO: 0.97 - 50% in AOO: 1.34 - undiluted test substance: 1.61 Lymph node weight - vehicle AOO: 1.00 - 25% in AOO: 1.20 - 50% in AOO: 1.37 - undiluted test substance: 1.79 Ear weight - vehicle AOO: 1.00 - 25% in AOO: 1.08 - 50% in AOO: 1.18 - undiluted test substance: 1.25
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: DPM / lymph node pair - vehicle AOO: 348-0 - 25% in AOO: 665.9 - 50% in AOO: 1,123.4 - undiluted test substance: 1,844.1
Any other information on results incl. tables
Body weight [g] on day 0 and day 5
- vehicle AOO: 20.1/20.3
- 25% in AOO: 20.2/21.1
- 50% in AOO: 20.2/20.3
- undiluted test substance: 19.9/20.4
Flow cytometry
The following tables show the mean values and the ratio of treated groups divided by concurrent control (SI) of the respective parameter with values > 1.25 in bold.
Mean values
LY LLN GIGA/L | B220 LLN % LY | B/T LLN % | CD3 LLN % LY | CD4 LLN % CD3 | CD8 LLN % CD3 | CD 19 LLN % LY | |
AOO | 1.24 | 8.75 | 9.91 | 91.01 | 55.53 | 30.5 | 7.44 |
25% in AOO | 1.2 | 8.2 | 10.05 | 90.88 | 57.94 | 30.66 | 6.71 |
50% in AOO | 1.66** | 8.78 | 10.37 | 90.61 | 56.34 | 31.65 | 7.07 |
undiluted (1) | 2.00** | 9.02 | 15.37* | 86.71* | 54.69 | 28.67 | 7.33 |
Kruskal-Wallis + Wilcoxon-test, two sided, *p<= 0.05; **p<=0.01 |
Stimulation indices
LY LLN GIGA/L | B220 LLN % LY | B/T LLN % | CD3 LLN % LY | CD4 LLN % CD3 | CD8 LLN % CD3 | CD 19 LLN % LY | |
AOO | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
25% in AOO | 0.97 | 0.94 | 1.01 | 1 | 1.04 | 1.01 | 0.9 |
50% in AOO | 1.34 | 1 | 1.05 | 1 | 1.01 | 1.04 | 0.95 |
undiluted (1) | 1.61 | 1.03 | 1.55 | 0.95 | 0.98 | 0.94 | 0.99 |
(1) Calculation of B/T, CD3, CD4 and CD8 on basis of 4 animals due to outlier values. |
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Based on the results of this study, the test substance chlorobenzene does not nedd to be classified for skin sinsitization according to Regulation (EU) No. 1272/2008 and Directive 67/548/EEC.
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