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EC number: 203-628-5 | CAS number: 108-90-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1960
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented study report which meets basic scientific principles.
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 960
- Report date:
- 1960
Materials and methods
- Principles of method if other than guideline:
- BASF test: A single dose of the test substance was injected in to the peritoneal cavity of the animals. After application, the animals were observed for mortality and clinical signs of toxicity. Animals that died during the experiment were necropsied. Surviving animals were sacrificed one week after application for the purpose of necropsy.
- GLP compliance:
- no
- Remarks:
- - GLP was not mandatory at the time when study was performed.
Test material
- Reference substance name:
- Chlorobenzene
- EC Number:
- 203-628-5
- EC Name:
- Chlorobenzene
- Cas Number:
- 108-90-7
- Molecular formula:
- C6H5Cl
- IUPAC Name:
- chlorobenzene
- Details on test material:
- Name: Chlorbenzol
Purity: no data
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: Tübinger
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Designation: Tübinger maeuse
- Weight range at study initiation: 18.5 - 31g
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- olive oil
- Details on exposure:
- The test substance was administered as a 2%, 5% and 10% solution in olive oil. Application volume was between 8 - 25 ml/kg bw.
- Doses:
- 0.20; 0.40; 0.80; 1.25; 1.60 ml/kg bw (222.5; 445; 890; 1,390.6; 1,780 mg/ml)
- No. of animals per sex per dose:
- 0.20 ml/kg bw: 5 animals
0.40 ml/kg bw: 10 animals
0.80 ml/kg bw: 10 animals
1.25 ml/kg bw': 5 animals
1.60 ml/kg bw: 5 animals - Control animals:
- no
- Statistics:
- On the basis of observed lethality, the LD50 was estimated using graphical evaluation of the dose response curve on probability paper.
Results and discussion
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 890 mg/kg bw
- Remarks on result:
- other: Corresponds to approx. 0.80 ml/kg bw
- Mortality:
- A concentration dependency of mortality was observed.
- 0.2 ml/kg bw: 0/5
- 0.4 ml/kg bw: 0/5
- 0.8 ml/kg bw: 5/10 (death occured between 48-72h).
- 1.25 ml/kg bw: 7/10 (6 animals within 48h. 7th animals within 72h).
- 1.6 ml/kg bw: 5/5 (4 animals died within 20h after application. 5th animal between 48h and 72h. - Clinical signs:
- - 0.2 ml/kg bw: Shabby fur, adhered eyelids.
- 0.4 ml/kg bw: Agitatation on day of application, otherwise, no other abnormality was detected.
- 0.8 ml/kg bw: On the day of application, the animals were groggy, very agitated and had accelerated irregular respiration. On the following day, the animals had a shabby fur, partially adhered eyelids and displayed grogginess and apathy.
- 1.25 ml/kg bw: On day of application, the animals experienced excitement, tremor, grogginess and slight abdominal position. On the following day, a shabby fur glued together by the test substance was observed.
- 1.6ml/kg bw: 5 minutes after application, grogginess and severe tremor was observed. After 20 mins, abdominal positioning partly merged with lateral positioning was observed. 2 1/2 h post application, the symptoms still persisted. In addition, accelerated respiration was also observed. 20 h post application, following symptoms were still observable in the surviving animals: severe tremor, adhered eyes, oily fur, intermittent respiratation, grogginess and slight abdorminal position. In the following days, the fur of the animals were shabby and glued with the test substance. - Gross pathology:
- The following findings reported were observed only in those animals that perished in the study. No abnormality was found in the organs (liver, kidney, spleen) of the animals that were sacrificed one week after application of the test substance.
0.8 ml/kg bw (5 deaths): slight liver decolouration (2/5), reddened small intestine (2/5), kidney decolouration (1/5)
1.25 ml/kg bw: (7 deaths): liver (light red), reddened small intestine, decolouration of kidney (6/7)
1.6ml/kg bw: (5 deaths): slightly reddened small intestine (1/5), decolouration of organs (3/5)
Applicant's summary and conclusion
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