Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2016

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)
GLP compliance:
no
Type of method:
shake-flask method to: flask method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Analytical method:
high-performance liquid chromatography

Results and discussion

Partition coefficient
Type:
log Pow
Partition coefficient:
-2.57
Temp.:
23 °C
pH:
> 8.2 - < 8.6

Any other information on results incl. tables

Determination stock solution n-octanol test substance test substance LogPow

No [ml] [ml] conc. in water[mg/L] conc. in octanol [mg/L]

A1 5 10 783 2.05 -2.60

A2 5 10 813 2.16 -2.56

B1 10 10 792 2.12 -2.58

B2 10 10 796 2.15 -2.57

C1 20 10 799 2.26 -2.54

C2 20 10 778 2.22 -2.56

mean -2.57

HPLC conditions:

Column : Ascentis Express C18, 150 mm x 4,6 mm ID, 2,7 μm,

Eluent A : 0,05 mol/l phosphoric acid / acetonitrile = 95/5 (v/v)

Eluent B : acetonitrile

Gradient : 0 min 0 % B

: 10 min 90 % B

: 15 min 0 % B

Flow rate : 1 ml/min

Temperature : 40°C

Detector : UV, 200 nm

Inj. Volume : 5 μl (water phases) and 20 μl (n-octanol phases)

Recovery rate: 98 - 103%

Applicant's summary and conclusion

Conclusions:
The Log Pow of the substance is -2.57 at 23°C.
Executive summary:

The Partition Coefficient n-octanol/water of the substance was determined according to OECD 107 with the shake flask method and was found to be -2.57 at 23°C.