Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 944-817-9 | CAS number: 244626-73-1
In this in vitro assessment of the mutagenic potential of the test item, histidine-dependent auxotrophic mutants of Salmonella typhimurium, strains TA1535, TA1537, TA98 and TA100, and a tryptophan-dependent mutant of Escherichia coli, strain WP2 uvrA (pKM101), were exposed to the test material diluted in dimethyl sulphoxide (DMSO). DMSO was also used as a vehicle control. The study was conducted in compliance with OECD 471, EC B.13/14 and OPPTS 870.5100 test guidelines and with GLP.
Two independent mutation tests were performed in the presence and absence of liver preparations (S9 mix) from rats treated with phenobarbital and 5,6-benzoflavone. The first test was a standard plate incorporation assay; the second included a pre-incubation stage.
Concentrations of the test material up to, and including, 5000 µg/plate were tested. This is the standard limit concentration recommended in the regulatory guidelines that this assay follows. Other concentrations used were a series ofcahalf-log10dilutions of the highest concentration.
No signs of toxicity towards the tester strains were observed in either mutation test following exposure to the test material.
No evidence of mutagenic activity was seen at any concentration of the test material in either mutation test.
The concurrent positive controls verified the sensitivity of the assay and the metabolising activity of the liver preparations. The mean revertant colony counts for the vehicle controls were within or close to the current historical control range for the laboratory.
It was concluded that the test material showed no evidence of mutagenic activity in this bacterial system under the test conditions employed.
This study is considered as acceptable and satisfies the requirement for the bacterial gene mutation endpoint.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again