1,2-dichloro-3-nitrobenzene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

as part of an OECD guideline 204 study

Deviations:

yes

Remarks:

1.) photoperiod 14 to 10 hours instead of 12 to 16 hours, 2.) no daily measurement of pH and DOC, 3.) origin and purity of test substance not reported

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

- number of test concentrations: 5
- Controls: one control without test substance and one control containing the highest concentration of the vehicle acteone used (158 mg/l)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Strain: Hamilton-Buchanan
- Source: Doller Zierfischzüchterei, 5950 Finnentrop-Serkenrode, Germany
- Length at study initiation (length definition, mean, range and SD): 2.0 +/- 1.0 cm
- Feeding during test: none

ACCLIMATION
- Acclimation period: at least 12 days before starting the test
- Type and amount of food: daily feeding with TetraMin

=> only healthy fish were tested

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23 +/- 1 °C

pH:

8.15 +/- 0.2

Dissolved oxygen:

oxygen saturation in the basin connected upstream to the test basin => test basins were not ventilated due to the volatile character of the test substance

Nominal and measured concentrations:

basin 0 control
basin 1 - n: 12.5 mg/l; a: 13.4 mg/l
basin 2 - n: 6.25 mg/l; a: 6.28 mg/l
basin 3 - n: 3.13 mg/l; a: 2.82 mg/l
basin 4 - n: 1.57 mg/l; a: 1.59 mg/l
basin 5 - n: 0.78 mg/l; a: 0.74 mg/l
basin 6 solvent control

Details on test conditions:

TEST SYSTEM
- Type: closed 10 l aquarium
- Aeration: oxygen saturation in the basin connected upstream to the test basin => test basins were not ventilated due to the volatile character of the test substance
- No. of organisms per vessel: 10

OTHER TEST CONDITIONS
- Photoperiod: 14 -10 hours

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

2.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

6.28 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

3.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

6.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Sublethal observations / clinical signs:

recovery rate of 2,3 -dichloronitrobenzene: 80.2 % (± 5.7)

The mortality in the control basins was 0% in all cases. Observations were made after 48 h and 96 h and the observed results on mortality were the same for both time points as outlined in the table below:

basin	analytical concentration [mg/L]	number of animals (total)	number of dead animals
1	13.4	10	10
2	6.28	10	10
3	2.60	10	0
4	1.59	10	0
5	0.74	10	0

Validity criteria fulfilled:

yes

Remarks:

validity criteria were fulfilled during the test: - mortality in the control basins was 0% in all cases - analytical measurement of test concentrations - Aeration: oxygen saturation in the basin connected upstream to the test basin

Conclusions:

The short-term toxicity to fish (zebrafish, Danio rerio) was investigated in a flow-through fresh-water system over a period of 96 h. The following effect concentrations (based on nominal test material concentration) LC50: 4.4 mg/L; Based on measured concentration the LC50 is 3.8 mg/L

Executive summary:

The short-term toxicity to fish (zebrafish, Danio rerio) was investigated in a flow-through fresh-water system over a period of 96 h. The following effect concentrations (based on nominal test material concentration) were found:

LC0: 3.1 mg/L

LC50: 4.4 mg/L

LC100: 6.25 mg/L

Based on measured concentration the LC50 is 3.8 mg/L

Description of key information

For transported isolated intermediates according to Reach, Annex XVIII, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".

 

The short-term toxicity to fish (zebrafish, Danio rerio) was investigated in a flow-through fresh-water system over a period of 96 h. The following effect concentrations (based on nominal test material concentration) were found:

LC0: 3.1 mg/L

LC50: 4.4 mg/L

LC100: 6.25 mg/L

Based on measured concentration the LC50 is 3.8 mg/L.

 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

3.8 mg/L