Methyl anthranilate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

To determine the toxicity of test chemical to microoganisms for 24 hours by Liquid Culture method.

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

- Samples were prepared as 10% (w/v) solutions in 95% (v/v) ethanol.
- In all experiments appropriate solvent controls were included. Antimicrobial chemicals included as controls were: TCC, Monsanto; Irgasan DP 300, Clba-Geigy; and hexachlorophene.

Stock cultures:
- Stock cultures were maintained on agar slants composed of tryptone (Difco), 0.3%; yeast extract (Difco), 0.3%; glucose, 0.9 K2HPO 4, 0.1%, and agar, 1% (referred to as TGY agar). Slants for the diphtheroid strain were supplemented with 0.5% Tween 80 (ICI America, Inc.).

Test media:
- TGY medium was used without the agar, and the pH was not adjusted (pH 7.0) in order to optimize growth of all the test organisms.
- In this case the diphtheroid medium was supplemented with 0.1% Tween 80.

Vehicle:

yes

Test organisms (species):

other: Staphylococcus aureus, Escherichia coli, Candida albicans and diphtheroid

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

pH:

7.0

Nominal and measured concentrations:

Nominal concentrations: 0.1, 1.0, 10, 100, and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: broth cultures (18 x 150 mm tubes; 10.0 ml/tube).

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

other: MIC

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: for S. aureus and E.coli

Duration:

24 h

Dose descriptor:

other: MIC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: for C. albicans and Diphtheroid

Details on results:

- Control antimicrobials were tested in the range 0.02 to 200 ppm depending on the chemical and the organism.

Validity criteria fulfilled:

not specified

Conclusions:

The Minimum Inhibitory Concentration of the test chemical for 24 h on Staphylococcus aureus, Escherichia coli and Candida albicans, diphtheroid was determined to be >1000mg/L and 1000mg/L, respectively.

Executive summary:

A study was conducted to determine the minimum inhibition concentration on microorganisms for 24h. Organisms used for the study were Staphylococcus aureus, Escherichia coli, Candida albicans and diphtheroid, which were obtained from Monmouth Medical Center, Long Branch, New Jersey and the Department of Dermatology, University of Pennsylvania, respectively. Nominal concentrations used were 0.1, 1.0, 10, 100, and 1000 mg/L. MIC was determined by Liquid Culture method in which TGY medium was used without the agar, while the pH was 7.0. Thus, The Minimum Inhibitory Concentration of the test chemical for 24 h on Staphylococcus aureus, Escherichia coli and Candida albicans, diphtheroid was determined to be >1000mg/L and 1000mg/L, respectively.

Description of key information

A study was conducted to determine the minimum inhibition concentration on microorganisms for 24h. Organisms used for the study were Staphylococcus aureus, Escherichia coli, Candida albicans and diphtheroid, which were obtained from Monmouth Medical Center, Long Branch, New Jersey and the Department of Dermatology, University of Pennsylvania, respectively. Nominal concentrations used were 0.1, 1.0, 10, 100, and 1000 mg/L. MIC was determined by Liquid Culture method in which TGY medium was used without the agar, while the pH was 7.0. Thus, The Minimum Inhibitory Concentration of the test chemical for 24 h on Staphylococcus aureus, Escherichia coli and Candida albicans, diphtheroid was determined to be >1000mg/L and 1000mg/L, respectively.

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

Additional information

Based on the experimental data for the target as well as read-across analogues which are extracted by using mechanistic approach and functionally and structurally similar to the target chemical toxicity of test chemical was determined on the basis of growth inhibition of microorganisms. The studies are summarized as below:

 

A study was conducted to determine the minimum inhibition concentration on microorganisms for 24h. Organisms used for the study were Staphylococcus aureus, Escherichia coli, Candida albicans and diphtheroid, which were obtained from Monmouth Medical Center, Long Branch, New Jersey and the Department of Dermatology, University of Pennsylvania, respectively. Nominal concentrations used were 0.1, 1.0, 10, 100, and 1000 mg/L. MIC was determined by Liquid Culture method in which TGY medium was used without the agar, while the pH was 7.0. Thus, The Minimum Inhibitory Concentration of the test chemical for 24 h on Staphylococcus aureus, Escherichia coli and Candida albicans, diphtheroid was determined to be >1000mg/L and 1000mg/L, respectively. 

 

A study was conducted to determine the toxicity of test chemical on microorganisms for 24h. Organisms used for the study were Staphylococcus aureus, Escherichia coli, Candida albicans and diphtheroid, which were obtained from Monmouth Medical Center, Long Branch, New Jersey and the Department of Dermatology, University of Pennsylvania, respectively. Petri Plate-Paper Disc method was used, wherein, TGY agar was used. The Paper discs of were soaked with 20µl of the 10% test solutions. Thus, the zone diameter of the test chemical for 24 h on Escherichia coli, Staphylococcus aureus, Candida albicans and diphtheroid by Petri Plate-Paper Disc method was determined to be 14mm, 12mm, 16mm and 0mm respectively.

 

A study was conducted to determine the toxicity of test chemical on Pseudomonas putida for 16h. Study was performed according to ISO 10712 (Water quality – Pseudomonas putida growth inhibition test (Pseudomonas cell multiplication inhibition test. After the exposure of test chemical for 16 h, the EC50 and EC10 value were determined to be 380mg/L and 140mg/L, respectively