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Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to guideline
Guideline:
other: Refer below principle
Principles of method if other than guideline:
In the study the decoloration of textile dye Cibacron Brilliant Yellow 3B-A and their effect on toxicity on Vibrio fischeri are evaluated by Microtox assay.
GLP compliance:
not specified
Specific details on test material used for the study:
- IUPAC name: trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate
- Name of test material (as cited in study report): Cibacron Brilliant Yellow 3B-A
- Molecular formula : C25H18Cl3N9O10S3.3Na
- Molecular weight : 872.974 g/mol
- Smiles notation : c1(\N=N\c2c(n(c3cc(c(S([O-])(=O)=O)cc3Cl)Cl)nc2C)O)cc(ccc1S(=O)(=O)[O-])Nc1nc(nc(n1)Cl)Nc1ccc(cc1)S(=O)(=O)[O-].[Na+].[Na+].[Na+]
- InChl : 1S/C25H18Cl3N9O10S3.3Na/c1-11-21(22(38)37(36-11)18-9-16(27)20(10-15(18)26)50(45,46)47)35-34-17-8-13(4-7-19(17)49(42,43)44)30-25-32-23(28)31-24(33-25)29-12-2-5-14(6-3-12)48(39,40)41;;;/h2-10,38H,1H3,(H,39,40,41)(H,42,43,44)(H,45,46,47)(H2,29,30,31,32,33);;;/q;3*+1/p-3
- Substance type:Organic
- Physical state: Solid
Analytical monitoring:
yes
Details on sampling:
Eight serial dilutions was used in study.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls:1 g ZnSO4 · 7H2O l−1 was used as the positive control and 1 g glucose l−1 as the negative control.
Test organisms (species):
Vibrio fisheri
Details on inoculum:
Frozen sample was brought to room temperature and centrifuged.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
5 min
Hardness:
No data
Test temperature:
room temperature
pH:
6
Dissolved oxygen:
No data
Salinity:
No data
Conductivity:
No data
Details on test conditions:
TEST SYSTEM
- Test vessel:Erlenmeyer shake flasks
- No. of vessels per concentration (replicates):triplicate

OTHER TEST CONDITIONS
- Adjustment of pH:The pH of the sample was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH.
Reference substance (positive control):
not specified
Key result
Duration:
5 min
Dose descriptor:
other: EC20
Effect conc.:
> 75 other: %
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
other: 20% decrease of bioluminescence in the Microtox assay
Remarks on result:
other: EC20 is the percent dilution of the sample (v/v)
Details on results:
EC20 reported is the average of triplicate analysis.
Validity criteria fulfilled:
not specified
Conclusions:
From Microtox assay the test substance trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate i.e Cibacron Brilliant Yellow 3B-A was moderately non-toxic to micro organism Vibrio fischeri on the basis of the percent concentration to decrease 20% of the luminescence of Vibrio fischeri (EC20).
Executive summary:

In the study effect on toxicity on Vibrio fischeri are evaluated by Microtox assay for textile dye Cibacron Brilliant Yellow 3B-A (IUPAC name- trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate).

 

Frozen samples were brought to room temperature,and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples according to the test protocols defined by the manufacturer (Azur Environmental, Newark, Del.).From eight serial dilutions,the percent concentration to decrease 20% of the luminescence of amodified strain of Vibrio fischeri (EC20) after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software (1999) Azur Environmental, Newark, Del.]. A solution of 1 g ZnSO4 · 7H2O l−1 was used as the positive control and 1 g glucose l−1 as the negative control. Each EC20 reported is the average of triplicate analysis.

 

As per the criteria (the percent concentration to decrease 20% of the luminescence of Vibrio fischeri EC20))

>100% = not toxic

100%> EC20 > 75% = moderately non-toxic

75% > EC20 > 50% = toxic

50% > EC20 > 25% = moderately toxic

 

And test chemical Cibacron Brilliant Yellow 3B-A gives the percent concentration to decrease 20% of the luminescence of Vibrio fischeri (EC20) was 100%> EC20 > 75% thus it is concluded that the textile dye Cibacron Brilliant Yellow 3B-A was observed to be moderately non-toxic to micro organism Vibrio fischeri.

Description of key information

From Microtox assay the test substance  trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate i.e Cibacron Brilliant Yellow 3B-A was moderately non-toxic to micro organism Vibrio fischeri  on the basis of the percent concentration to decrease 20% of the luminescence of Vibrio fischeri (EC20).

Key value for chemical safety assessment

Additional information

In the study effect on toxicity on Vibrio fischeri are evaluated by Microtox assay for textile dye Cibacron Brilliant Yellow 3B-A (IUPAC name- trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate).

 

Frozen samples were brought to room temperature,and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples according to the test protocols defined by the manufacturer (Azur Environmental, Newark, Del.).From eight serial dilutions,the percent concentration to decrease 20% of the luminescence of amodified strain of Vibrio fischeri (EC20) after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software (1999) Azur Environmental, Newark, Del.]. A solution of 1 g ZnSO4 · 7H2O l−1 was used as the positive control and 1 g glucose l−1 as the negative control. Each EC20 reported is the average of triplicate analysis.

 

As per the criteria (the percent concentration to decrease 20% of the luminescence of Vibrio fischeri EC20))

>100% = not toxic

100%> EC20 > 75% = moderately non-toxic

75% > EC20 > 50% = toxic

50% > EC20 > 25% = moderately toxic

 

And test chemical Cibacron Brilliant Yellow 3B-A gives the percent concentration to decrease 20% of the luminescence of Vibrio fischeri (EC20) was 100%> EC20 > 75% thus it is concluded that the textile dye Cibacron Brilliant Yellow 3B-A was observed to be moderately non-toxic to micro organism Vibrio fischeri.