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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Remarks:
no GLP and no analytics were performed, limited documentation.
Qualifier:
according to
Guideline:
other: DIN 38412 Part 9, Bestimmung der Hemmwirkung von Wasserinhaltsstoffen auf Gruenalgen
Principles of method if other than guideline:
Method: Scenedesmus cell multiplication inhibition test
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
23 (±2°C)
pH:
7.6-10.4
Nominal and measured concentrations:
Nominal concentrations: 31.25, 62.50, 125, 250, 500 mg/L + control
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass, 10 ml
- Aeration: no
- Control end cells density: 10000
- No. of vessels per concentration (replicates): no data
- No. of vessels per control (replicates): no data


GROWTH MEDIUM
- Standard medium used: yes, DIN 38412 Part 9


OTHER TEST CONDITIONS
- Light intensity and quality: 120 µE m-2 s-1


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter

Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
96 h
Dose descriptor:
EC90
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
96 h
Dose descriptor:
EC20
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

At the exposure period of 72 h: 

EC20 = 500 mg/L

EC50 > 500 mg/L

EC90 > 500 mg/L 

Inhibition at 72 h was 20 % at 500 mg/L and 11 % at 250 mg/L. At 96 h no inhibition was observed, biomass increased by 10 % at 500 mg/L and 9 % at 250mg/L compared to the control.

Description of key information

With high probability acutely not harmful to algae.

Key value for chemical safety assessment

Additional information

Acute toxicity of IBDU to the green alga Scenedesmus subspicatus was determined according to the German Industrial Standard Guideline DIN 38412, part 9, using five test concentrations ranging from 31.25 to 500 mg/l (nominal) and an untreated control. The ErC50 (72 h) for the endpoint growth rate was > 500 mg/l (nominal) (BASF AG 1990).