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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21 May 1986 - 23 May 1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
no GLP and no analytics were performed
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The substance was powdered in a mortar for 1 min at 293 Kelvin. The concentration of the test substance in the stock solution was 500 mg/L. The stock solution was stirred for 30 min at 293 Kelvin. A serial dilution in dilution water was prepared. To each test vessel 10 ml of the respective dilution were added.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978
- Age at study initiation (mean and range, SD): <24h
- Method of breeding: comparable to test conditions, Feed: Whilst changing water Brewer's yeast was given, also
1 x daily grown Green algae culture
- Feeding during test: no

ACCLIMATION
- Acclimation conditions (same as test or not): yes
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.7 ± 0.5 mmol/L
Test temperature:
292-294 Kelvin
pH:
7.84-8.08
Dissolved oxygen:
7.74-8.86 mg/L
Nominal and measured concentrations:
Nominal concentrations: 62.5, 125, 250, 500 mg/L and control
Details on test conditions:
TEST SYSTEM
- Test vessel: reagent tubes with flat bottom
- Material, size, headspace, fill volume: glas, 10ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water, purified by charcoal to remove chlorine and filtered through 6 µm filter
- Dilution water chemistry: according to the test guideline DIN 38412, Part 11
The properties of the adjusted water as follows:
Total water hardness: 2.70 +/- 0.5 mmol/l
Acidity constant - pH 4.3: 0.80 +- 0.1 mmol/l
Molar ratio: Ca:Mg ca. 4 :1
Oxygen content > 7 mg/l


OTHER TEST CONDITIONS
- Light intensity: no light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations of swimming inability after 24 and 48 hours.

Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
250 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Conc. [mg/l]     Immobile daphnids after
0h 24h 48h

Control 0 0 0
62.5 0 0 0
125 0 0 0
250 0 0 2
500 0 2 10
In the stock solution and in the test vessels test substance was visible  over the whole test period



Description of key information

With high probability acute not harmful to aquatic invertebrates

Key value for chemical safety assessment

Additional information

A non GLP study on the acute toxicity of IBDU to Daphnia magna was performed according to the Directive 84/449/EEC, C. 2 in a static test system. For a test period of 48 hours an EC50 value of 500 mg/l was reported (BASF AG 1988).