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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
13. - 17 Oct 1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
No information on stability of test substance under test conditions available, no analytical monitoring.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1984
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Preparation of the test substance: the substance was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria.
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Forellenhof Fredelsloh, Dr . med. vet. Otto Mueller, D-3413 Moringen 1, FRG
- Length at study initiation (length definition, mean, range and SD): 5.7 cm (4.9 - 6.5 cm)
- Weight at study initiation (mean and range, SD): 2.2 g (1.4 - 3.4 g)
- Feeding during test: no


ACCLIMATION
- Acclimation period: 10 weeks
- Acclimation conditions (same as test or not): no, flow though
- Type and amount of food: Growing feef F/B 50, SSNIEF Spezialdiaeten GmbH, D-4770 Soest, Frg, ad libitum
- Health during acclimation (any mortality observed): no mortalities
- Medical treatment: once with 10 mg/L Tetracycline hydrochloride, twice with 0.05 mg/L Malachite green chloride
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
2.5 mmol/L
Test temperature:
12 °C (± 0°C)
pH:
8.3 (8.1-8.6)
Dissolved oxygen:
9.7-11.4 mg/L
Nominal and measured concentrations:
Nominal concentration tested: 1000 mg/L (this selection was based on the results of a range finding study which resulted in a LC50 after 96 hours of 1000 mg/L). No analytical dose verification.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass aquarium with a stainless steel frame (80 cm x 35 cm x 46 cm)
- Aeration: yes, slight
- No. of organisms per vessel: 10
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.22 g/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater according DIN 34812, Part 11, Draft Sept. 1981
Preparation from fully demineralized tap water
- Ca/mg ratio: 4:1
- Conductivity: max. 10 micro MHO


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, abnormal symptoms
Determination of mortality and symptoms: at 1, 4, 24, 48, 72, 96 hours.


TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: selection was based on the results of a range finding study which resulted in a LC50 after 96 hours of 1000 mg/L
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Mortality and behaviour
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: none
- Mortality of control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Undissolved test substance was visible on the water surface and on the bottom of the aquarium.

No symptoms were noted. No mortality.

Description of key information

With high probability acute not harmful to fish.

Key value for chemical safety assessment

Additional information

In a non GLP study on the acute toxicity to fish (static, performed similar to OECD guideline 203), no mortalities were observed up to 1000 mg/l IBDU (LC50(96 h) > 1000 mg/l, nominal). Salmo gairdneri was used as test organism. The substance (1000 mg/l) was added to the test water without any pretreatment. Undissolved test substance was visible on the water surface and on the bottom of the aquarium.