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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
13. - 17 Oct 1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
No information on stability of test substance under test conditions available, no analytical monitoring.
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1984
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Preparation of the test substance: the substance was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria.
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Forellenhof Fredelsloh, Dr . med. vet. Otto Mueller, D-3413 Moringen 1, FRG
- Length at study initiation (length definition, mean, range and SD): 5.7 cm (4.9 - 6.5 cm)
- Weight at study initiation (mean and range, SD): 2.2 g (1.4 - 3.4 g)
- Feeding during test: no


ACCLIMATION
- Acclimation period: 10 weeks
- Acclimation conditions (same as test or not): no, flow though
- Type and amount of food: Growing feef F/B 50, SSNIEF Spezialdiaeten GmbH, D-4770 Soest, Frg, ad libitum
- Health during acclimation (any mortality observed): no mortalities
- Medical treatment: once with 10 mg/L Tetracycline hydrochloride, twice with 0.05 mg/L Malachite green chloride
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
2.5 mmol/L
Test temperature:
12 °C (± 0°C)
pH:
8.3 (8.1-8.6)
Dissolved oxygen:
9.7-11.4 mg/L
Nominal and measured concentrations:
Nominal concentration tested: 1000 mg/L (this selection was based on the results of a range finding study which resulted in a LC50 after 96 hours of 1000 mg/L). No analytical dose verification.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass aquarium with a stainless steel frame (80 cm x 35 cm x 46 cm)
- Aeration: yes, slight
- No. of organisms per vessel: 10
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.22 g/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater according DIN 34812, Part 11, Draft Sept. 1981
Preparation from fully demineralized tap water
- Ca/mg ratio: 4:1
- Conductivity: max. 10 micro MHO


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, abnormal symptoms
Determination of mortality and symptoms: at 1, 4, 24, 48, 72, 96 hours.


TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: selection was based on the results of a range finding study which resulted in a LC50 after 96 hours of 1000 mg/L
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Mortality and behaviour
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Behavioural abnormalities: none
- Mortality of control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Undissolved test substance was visible on the water surface and on the bottom of the aquarium.
Sublethal observations / clinical signs:

No symptoms were noted. No mortality.

Description of key information

With high probability acute not harmful to fish.

Key value for chemical safety assessment

Additional information

In a non GLP study on the acute toxicity to fish (static, performed similar to OECD guideline 203), no mortalities were observed up to 1000 mg/l IBDU (LC50(96 h) > 1000 mg/l, nominal). Salmo gairdneri was used as test organism. The substance (1000 mg/l) was added to the test water without any pretreatment. Undissolved test substance was visible on the water surface and on the bottom of the aquarium.