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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
aniline
Key result
Parameter:
% degradation (O2 consumption)
Value:
78
Sampling time:
28 d
Details on results:
Following results were reported.

In the test vessels N remained as NH3 form and after the cultivation and extents of biodegradation were calculated using ThOD based on NH3.

Biodegradation rates (28 days)
---------------------------------------------
by BOD 77% 81% 77% (Av. 78%)
by DOC 89% 90% 90% (Av. 90%)
by HPLC100% 100% 100% (Av. 68%)
---------------------------------------------

Based on the BOD analysis, the 10-day window was fulfilled.

After the 28 days of cultivation, pH remained within the required range of 6.0 to 8.5.

Oxygen consumption of the blank vessels was 14.3 mg/l.

Kinetic of test substance (in %):
= 72 after 7 day(s)
= 78 after 14 day(s)
= 78 after 21 day(s)
= 78 after 28 day(s)

Degradation products: not measured
Results with reference substance:
Kinetic of control substance (in %):
= 60 after 7 day(s)
= 72 after 14 day(s)
Interpretation of results:
readily biodegradable
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to
Guideline:
OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
Version / remarks:
1981
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
28 d
Initial conc.:
19.6 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Reference substance:
other: not reported
Key result
Parameter:
% degradation (DOC removal)
Value:
85
Sampling time:
28 d
Details on results:
other: biodegradable, but not "readily biodegradable" according to OECD criteria (ready test pass level reached, but not the 10 day window)

Kinetic of test substance (in %):
= 8 ... 14 after 7 day(s)
= 24 ... 30 after 14 day(s)
= 48 ... 75 after 21 day(s)
= 78 ... 91 after 28 day(s)
Degradation products: not measured
Interpretation of results:
readily biodegradable, but failing 10-day window

Description of key information

Readily biodegradable (according to OECD criteria)

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Two biodegradation tests on readily biodegradbility are available for IBDU. In a GLP study according to OECD guideline 301C, IBDU was degraded by 78% after 28 days (NITE 2002). The OECD criteria: 10 -day window and pass level >= 60% were fullfilled. Therefore, IBDU can be classified as readiliy biodegradable. This result is supported by a study from BASF AG (1988), where in a non GLP test according to Guideline 301 E, IBDU was degraded by 85% after 28 days. In this test, however, the OECD criteria of a 10 day window was not fullfilled and by thereby this result can only be used as additional information.