Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study was conducted before GLP and guidelines and thus report detail is lacking. However, the study was done at a reputable laboratory to the state of the art at the time.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1957

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Method is similar to an OECD 402. Test subjects had test article applied to the skin under an occlusive dressing for 24 hours followed by a 14-day observation period.
GLP compliance:
no
Test type:
other:
Limit test:
no

Test material

Constituent 1
Reference substance name:
iso-Butyronitrile
IUPAC Name:
iso-Butyronitrile
Constituent 2
Chemical structure
Reference substance name:
Isobutyronitrile
EC Number:
201-147-5
EC Name:
Isobutyronitrile
Cas Number:
78-82-0
Molecular formula:
C4H7N
IUPAC Name:
2-methylpropanenitrile

Test animals

Species:
guinea pig
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
None given

Administration / exposure

Type of coverage:
occlusive
Vehicle:
other: 90/10 Acetone/Corn oil
Details on dermal exposure:
Test article was applied neat under occlusive contitions to the test subjects.
Duration of exposure:
24 hours
Doses:
1) Undiluted (5 and 10 cc/kg)
2) 20% in 90/10 acetone/corn oil (5-20 cc/kg)
3) 5% in 90/10 acetone/corn oil (5-20 cc/kg)
Control animals:
no

Results and discussion

Effect levels
Key result
Dose descriptor:
LD50
Effect level:
< 20 other: cc/kg
Gross pathology:
Moderate edema, slight or no redness in lower doses. In highest dose, gross edema, moderate redness at edges, brown necrosis at edges.

Applicant's summary and conclusion

Interpretation of results:
very toxic
Remarks:
Migrated information Criteria used for interpretation of results: US CPSC / US OSHA
Conclusions:
The acute dermal LD50 was > 20 cc/kg as a 5% suspension.
Executive summary:

The acture dermal LD50 of iso-butyronitrile in guinea pigs was:

1) LD50 of < 5 cc/kg for undiluted test article.

2) < 5 cc/kg for 20% in 90/10 acetone/corn oil

3) > 20 cc/kg for 5% in 90/10 acetone/corn oil.