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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: other guideline: EC Directive 2000/32, B13/14 OECD 471, 1997
GLP compliance:
yes
Type of assay:
other: not available

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
436-710-6
EC Name:
-
Cas Number:
756-13-8
Molecular formula:
C6F12O
IUPAC Name:
1,1,1,2,2,4,5,5,5-nonafluoro-4-(trifluoromethyl)pentan-3-one

Method

Species / strain
Species / strain / cell type:
bacteria, other: Salmonella typhimurium (TA1535, TA1537, TA100 and TA98) Escherichia coli WP2uvrA
Metabolic activation system:
S9 liver homogenate of rat induced with Aroclor 1254.
Test concentrations with justification for top dose:
Concentration range in the main test (with metabolic activation): 10 ... 5000 μg/plate
Concentration range in the main test (without metabolic activation): 10 ... 5000 μg/plate
Vehicle / solvent:
Solvent: ethanol

Results and discussion

Test resultsopen allclose all
Species / strain:
other: as specified above
Metabolic activation:
with
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(> 5000 μg/plate)
Species / strain:
other: as specified above
Metabolic activation:
without
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(> 5000 μg/plate)
Species / strain:
other: as specified above
Metabolic activation:
with
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(> 5000 μg/plate)
Species / strain:
other: as specified above
Metabolic activation:
without
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(> 5000 μg/plate)
Additional information on results:
Observations:
The bacterial background lawn was not reduced at all concentrations tested and no biologically relevant decrease in the number of revertants was observed. The presence of 5 and 10% (v/v) liver microsomal activation did not influence these findings. T-7479 did not induce a dose-related, two-fold increase in the number of revertant (His+) colonies in each of the four tester strains (TA1535, TA1537, TA98 and TA100) and in the number of revertant (Trp+) colonies in tester strain WP2uvrA both in the absence and presence of S9-metabolic activation. These results were confirmed in an independently repeated experiment.
Remarks on result:
other: Test system: preliminary test

Applicant's summary and conclusion

Conclusions:
Interpretation of results
negative with metabolic activation
negative without metabolic activation