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EC number: 300-340-2 | CAS number: 93925-38-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-08-10 to 1992-12-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: direct inclusion of test material to test vessels- Evidence of undissolved material (e.g. precipitate, surface film, etc): none stated
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Obtained from the Cottonwood Subdivision Wastewater Treatment Plant located in Franklin, Tennessee.One gallon of sludge collected on day of use.Sludge immediately aerated with low presure oil-free air and fed synthetc sewage feed at a rate of 50 mL/L.Triplicate 10 mL samples of the mixed sludge were dried at 100°C in a Fisherbrand drying oven, until a constant weight was achieved. Based on these results the sludge was diluted to produce a dry weight per unit volume of 4 g/L.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
- Hardness:
- Not applicable
- Test temperature:
- 20°C
- pH:
- not stated
- Dissolved oxygen:
- Initial dissolved oxygen concentration not specified.
- Salinity:
- not applicable
- Nominal and measured concentrations:
- 1000 mg/L (stated as ppm)
- Details on test conditions:
- TEST SYSTEM- Test vessel:glass serum bottle- Type (delete if not applicable): open - Material, size, headspace, fill volume: 1L, BOD bottle oxygen probe used to eliminate airspace- Aeration: yes (oil-free low pressure air delivered using a glass pipet)- No. of organisms per vessel: 4g/L dry weight sludge - 200 mL innoculm added per vessel- No. of vessels per concentration (replicates): 2- No. of vessels per control (replicates): 2 (one bfore and one after test material and reference runs started)- No. of vessels per reference (replicates): 3 (0.5 mL, 5 mL and 50 mL)TEST MEDIUM Synthetic sewage mediumOTHER TEST CONDITIONS- Adjustment of pH: NoEFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Every 10 minutes or until a linear trace covering sufficient range of oxygen concentrations was obtained.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (97% pure)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None specified- Effect concentrations exceeding solubility of substance in test medium: Yes, test concentrations higher than solubility.Replicate A of the sludge exposed to the test material showed a consumption of 0.48 mg O2/L over a period of 0.5 min; a consumption rate equal to 57.6 mg O2/L per hour. Replicate B showed a a consumption of 2.4 mg O2/L over a period of 3.17 min; a consumption rate equal to 45.8 mg O2/L per hour. Expressed as an inhibition of control values the two replicates produced inhibitions of -7.4% and 14.6% respectively.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes- Relevant effect levels: 50 ppm: 84.3% inhibition, 5 and 0.5 ppm: 10.5% inhibition. EC50 = 18.0 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effect of the test item on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC50 value of greater than 1000 mg/L. The No Observed Effect Concentration (NOEC) after 3 hours exposure was 1000 mg/L.
- Executive summary:
Introduction
A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals No. 209 "Activated Sludge, Respiration Inhibition Test".
Methods
Activated sewage sludge was exposed to an aqueous dispersion of the test item at a concentration of 1000 mg/L for a period of 3 hours at a temperature of 20 ± 2 deg. C with the addition of a synthetic sewage as a respiratory substrate. The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-dichlorophenol.
Results
The effect of the test item on the respiration of activated sewage sludge gave a 3-Hour EC50 value of greater than 1000 mg/L and a No Observed Effect Concentration (NOEC) of 1000 mg/L. The reference item gave a 3-Hour EC50 value of 18.0 mg/L.
Reference
Description of key information
The effect of the test item on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC50 value of greater than 1000 mg/L. The No Observed Effect Concentration (NOEC) after 3 hours exposure was 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals No. 209 "Activated Sludge, Respiration Inhibition Test".
Activated sewage sludge was exposed to an aqueous dispersion of the test item at a concentration of 1000 mg/L for a period of 3 hours at a temperature of 20 ± 2 deg. C with the addition of a synthetic sewage as a respiratory substrate. The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-dichlorophenol.
The effect of the test item on the respiration of activated sewage sludge gave a 3-Hour EC50 value of greater than 1000 mg/L and a No Observed Effect Concentration (NOEC) of 1000 mg/L. The reference item gave a 3-Hour EC50 value of 18.0 mg/L.
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