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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study according to guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
During this study samples of the test substance concentrations of nominal 6.25; 25; 100 mg/L M4 medium and the control (M4 medium) were taken after 0 h, 24 h and 48 h. Analytical determination showed no loss of test substance during the exposure period of 48 h. Recovery of test substance was between 104% and 118% of nominal test concentration.

Test solutions

Vehicle:
no
Details on test solutions:
The test concentrations were prepared by diluting a stock solution of 1 g/L. 200 mg test substance was weighed into a beaker, mixed with M4 medium and was stirred for 5 min. with a magnetic stirrer to dissolve the test substance in the test medium. Then the test substance preparation was filled into a graduated flask and made up to a total volume of 200 mL with M4 medium. The amounts corresponding to the test concentrations were pipetted into a graduated flask, made up to a total volume of 500 mL with M4 medium, and then transferred to the test vessels.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Test organisms were <24 hours old and bred at the testing facility. The daphnids were kept in M4 medium in a 16:8 hours light-dark cycle at a temperature of 20 +/- 2°C and were fed with singular algae.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20 +/- 2°C
pH:
tests start:8.4 - 9.6
test end: 8.4 - 8.5
Dissolved oxygen:
test start: 5.8 - 6.2
test end: 5.6 - 6.0
Salinity:
no data
Nominal and measured concentrations:
nominal: 6.25, 12.5, 25.0, 50.0 and 100 mg/L
Recovery of the test substance at test end (48h) was between 104% and 118% of nominal values.
Details on test conditions:
according to guideline
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
45.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL 37.9-55.6
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
EC50 (24h): 0.8 mg/l
Reported statistics and error estimates:
EC50 values, 95% confidence limits and NOEC were calculated with the ToxRat Professional software.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under presen conditions tested , a IC50 value of the test substance after 48h exposure of Daphnia magna is derived to be 45.9 mg/L (nominal). The test substance is stable in aqueous solution (recovery rates 104-118% of nominal).