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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

Based on the results of the tests, the toxicity threshold value (EC10) of .beta.-Cyclodextrin was more than 10000 mg!l.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
10 000 mg/L

Additional information

.Beta.-Cyclodextrin (99.5% pure) was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida according to DIN 38412 Part L8 and NEN-EN-ISO 10712. Suspensions of Pseudomonas putida were exposed to concentrations ranging from312.5to 10000 mg .beta.-Cyclodextrin per liter.

After incubation for 16 hours the extinction values of the bacterial cell suspensions were measured. No significant (significant: >10%) toxicity was observed. Based on the results of the test presently performed, the toxicity threshold value (EC10) of .beta.-Cyclodextrin was over 10000 mg/l. The EC50 value for the reference substance, 3,5-dichlorophenol, tested during the same experiment, was 25 mg/l. Since this value was within the accepted range of 10-30 mg/l for the EC50, and the multiplication factor of thebacteriawithin the test was over 60, it was concluded that the test conditions were optimal and the results obtained were valid.