3-[[3-(dimethylamino)propyl]amino]propiononitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-[[3-(dimethylamino)propyl]amino]propiononitrile

EC Number:

274-159-1

EC Name:

3-[[3-(dimethylamino)propyl]amino]propiononitrile

Cas Number:

69852-45-5

Molecular formula:

C8H17N3

IUPAC Name:

3-{[3-(dimethylamino)propyl]amino}propanenitrile

impurity 1

Reference substance name:

3,3'-[[3-(dimethylamino)propyl]imino]bispropiononitrile

EC Number:

275-331-9

EC Name:

3,3'-[[3-(dimethylamino)propyl]imino]bispropiononitrile

Cas Number:

71326-27-7

Molecular formula:

C11H20N4

IUPAC Name:

3,3'-{[3-(dimethylamino)propyl]imino}dipropanenitrile

Test material form:

liquid

Remarks:

colorless

Test animals

Species:

rat

Sex:

male/female

Details on test animals or test system and environmental conditions:

Healthy random bred rats of the Tif: RAIf (SPF) strain raised on our premises were used for these experiments. They were kept at a room temperature of 22 + 1° C, at a relative humidity of 55 + 5 % and on a 10 hours light cycle day. They received ad libitum rat food - NAFAG, Gossau SG - and water. Prior to treatment the animals were adapted to our laboratories for a minimum of 4 days and the initial body weight ranged from 160 to 180 grams.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 10, 20, 30, 50% of formulation

Doses:

1000, 2150, 2780, 3170, 3590 and 4640 mg/kg

No. of animals per sex per dose:

5

Control animals:

not specified

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 2 284 mg/kg bw

95% CL:

>= 2 026 - <= 2 576

Mortality:

Deaths occured after 24 hours at 2150 mg/kg dosage. 100% mortality was seen at 4649 mg/kg after 24 hours

Clinical signs:

Within 2 hours after treatment the rats in all dosage groups showed sedation, dyspnoea, exophthalmos, curved position and ruffled fur. Sedation became more accentuated as the dose was increased. The surviving animals recovered within 8 to 9 days.

Body weight:

Not recorded

Gross pathology:

No substance related gross organ changes were seen

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

The acute oral LD50 of TK 12271 in rats of both sexes observed over a period of 14 days is 2284 (2026-2576) mg/kg. The test material has therefore a slight acute toxicity to the rat by this route of administration.