2-bromoethylammonium bromide

Genetic toxicity: in vitro

Administrative data

Endpoint:

genetic toxicity in vitro, other

Remarks:

gene mutation study in yeast

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed publication

Data source

Reference

Reference Type:

publication

Title:

Testing of chemicals for mutagenic activity with Schizosaccharomyces pombe- A report of the U.S. Environmental Protection Agency Gene-Tox Program

Author:

N. Loprieno, R. Barale , E.S. Von Halle and R.C. yon Borstel

Year:

1983

Bibliographic source:

Mutation Research, 115 (1983) 215-223

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Reverse mutation was studied for the 2-Bromoethaneamine hydrobromide using yeast Schizosaccharomyces pombe

GLP compliance:

not specified

Type of assay:

other: Schizosaccharomyces pombe gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-bromoethylammonium bromide

EC Number:

219-924-2

EC Name:

2-bromoethylammonium bromide

Cas Number:

2576-47-8

Molecular formula:

C2H6BrN.BrH

IUPAC Name:

2-bromoethanaminium bromide

Test material form:

solid

Details on test material:

SMILES:BrCCN{+}.Br{-}

Specific details on test material used for the study:

- Name of test material: 2-Bromoethaneamine hydrobromide
- Molecular formula: C2H6BrNBrH
- Molecular weight: 204.892 g/mol
- Substance type: Organic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data

Method

Target gene:

his52, his7, met4, ade6 and ade7

Species / strain

Species / strain / cell type:

yeast, other: Schizosaccharomyces pombe strain Arg 1

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

No data

Metabolic activation:

without

Metabolic activation system:

Exogeneous metabolic activation system- S9 and S100

Test concentrations with justification for top dose:

No data

Vehicle / solvent:

No data

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

No data

Rationale for test conditions:

No data

Evaluation criteria:

The yeast cells were observed for reversion of mutation

Statistics:

No data

Results and discussion

Test results

Species / strain:

yeast, other: Schizosaccharomyces pombe strain Arg 1

Metabolic activation:

without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

No data

Any other information on results incl. tables

Table: Chemicals Tested for Mutagenicity on Stationary Phase Cells of Schizosaccharomyces pombe

Chemical	Strain	Metabolic activation	Genetic end point	Genetic activity
2-Bromoethaneamine hydrobromide	Arg 1	-	Reverse mutation	+

 

Applicant's summary and conclusion

Conclusions:

2-Bromoethaneamine hydrobromide induced reversion of mutation in yeast Schizosaccharomyces pombe and hence the chemical is likely to be mutagenic to yeast cells.

Executive summary:

Reverse mutation was studied for the 2-Bromoethaneamine hydrobromide using yeast Schizosaccharomyces pombe strain Arg 1. The study was performed without exogeneous metabolic activation system at either of the his52, his7, met4, ade6 and ade7 locus. All these systems allow the evaluation of back mutation induction at the original mutant site. 2-Bromoethaneamine hydrobromide induced reversion of mutation in yeast Schizosaccharomyces pombe without exogeneous metabolic activation system and hence the chemical is likely to be mutagenic to yeast cells.