m-acetylphenyl acetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-11-15 to 1999-12-10

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

m-acetylphenyl acetate

EC Number:

219-524-8

EC Name:

m-acetylphenyl acetate

Cas Number:

2454-35-5

Molecular formula:

C10H10O3

IUPAC Name:

3-acetylphenyl acetate

Test material form:

solid

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Source: supplied 1997 by the Umweltbundesamt, Institut für Wasser-, Boden- und
Lufhygiene, Berlin, Germany
- Age of parental stock (mean and range, SD): 8 - 23.5 hours

ACCLIMATION
- Acclimation period: for 8 hours under test conditions

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/L (=250.0 mg/L) as CaCO3

Test temperature:

21°C

pH:

pH 7.6 to 7.8

Dissolved oxygen:

at least 7.5 mg/L or higher

Nominal and measured concentrations:

- Nominal concentration: 4.6, 10, 21, 46, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass beaker
- No. of vessels per concentration (replicates): 20 Daphnia, divided into two groups of ten animals, each group in 50 mL test medium
- No. of vessels per control (replicates): 20 Daphnia, divided into two groups of ten animals, each group in 50 mL test medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water prepared according to guideline

OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark

RANGE-FINDING STUDY
- Test concentrations: not above 100 mg substance/L

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

90.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

21 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

21 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 7

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Results after 24 h and 48 h

Nominal concentration [mg/L]	Results after 24 h and 48 h
	Immobile daphnids				Oxygen content [mg/L) after 48 h	pH after 48 h
	Absolute		%
	24 h	48 h	24 h	48 h
0 (control)	0	0	0	0	8.1	7.8
4.6	0	0	0	0	8.1	7.8
10	0	0	0	0	8.0	7.8
21	0	0	0	0	8.0	7.8
46	0	2	0	10	7.8	7.7
100	6	15	30	75	7.7	7.6

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 was determined to be 90.9 mg/L.

Executive summary:

The 48 hour EC50 was calculated to be 90.9 mg test substance/L with 95% confidence limits from 51.7 to 159.6 mg/L, according to OECD TG 202 (1984).