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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Peer-reviewed assessment report (attached in section 13)
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
Qualifier:
according to
Guideline:
EPA OPP 72-1 (Fish Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Test type:
flow-through
Total exposure duration:
96 h
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
18 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
3.5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: Clinical signs
Conclusions:
The study was conducted according to OECD guideline 203 and EPA OPP 72-1 on ethoxyquin (CAS 91-53-2) itself. The study is specified in a draft assessment report and can be considered scientifically reasonable with no deficiencies in documentation or deviations from the guidelines. Hence, the validity criteria were met and the results can be considered as reliable to assess the toxicity of the test item towards rainbow trout.
Short term toxicity to fish was tested in a flow-through test design, the 96-hour LC50 value for rainbow trout exposed to ethoxyquin was 18 mg ethoxyquin/L based on mortality.
Executive summary:

The 96-hour LC50 value for rainbow trout exposed to ethoxyquin was 18 mg ethoxyquin/L. The 95 % confidence limits were 14 and 23 mg ethoxyquin/L. The no mortality concentration was 14 mg ethoxyquin/L. Based on the clinical signs of toxicity observed in the 7.0 and 14.0 mg ethoxyquin/L treatment groups, the NOEC was considered to be 3.5 mg ethoxyquin/ L.

Description of key information

Short-term toxicity to fish: LC50(96h) = 18 mg/L for rainbow trout (Oncorhynchus mykiss) (OECD TG 203, flow-through)

Key value for chemical safety assessment

LC50 for freshwater fish:
18 mg/L

Additional information

There is a valid, peer-reviewed OECD 203 guideline study without relevant deviations on ethoxyquin itself available, assessed with Klimisch 2. In this study, the following LC50 value was determined: 18 mg/L (96 h). The result can be considered as sufficiently reliable to assess the toxicity of ethoxyquin to fish (rainbow trout).

Based on the obtained result, the test item does not need to be classified as acute hazardous to the aquatic environment according to the Regulation (EC) No. 1272/2008.